Phylogeny and polymorphism in the E6 and E7 of human papillomavirus: alpha-9 (HPV16, 31, 33, 52, 58), alpha-5 (HPV51), alpha-6 (HPV53, 66), alpha-7 (HPV18, 39, 59, 68) and alpha-10 (HPV6, 44) in women from Shanghai,Infectious Agents and Cancer

当前位置： X-MOL 学术 › Infect. Agents Cancer › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Phylogeny and polymorphism in the E6 and E7 of human papillomavirus: alpha-9 (HPV16, 31, 33, 52, 58), alpha-5 (HPV51), alpha-6 (HPV53, 66), alpha-7 (HPV18, 39, 59, 68) and alpha-10 (HPV6, 44) in women from Shanghai
Infectious Agents and Cancer ( IF 3.7 ) Pub Date : 2019-11-21 , DOI: 10.1186/s13027-019-0250-9
Junwei Zhao ₁ , Qin Zhan ₂ , Junhan Guo ₁ , Min Liu ₁ , Yetian Ruan ₁ , Tailin Zhu ₃ , Lingfei Han ₂ , Fang Li ₁

Affiliation

BackgroundPersistent infection with human papillomaviruses (HPVs) has been associated with cervical intraepithelial neoplasia (CIN) and cervical cancer. However, why only a fraction of HPV cases progress to cancer is still unclear.MethodsWe focused on the heterogeneity, classification, evolution and dispersal of variants for 14 common HPV types in 262 HPV-positive patients with cervical lesions. The E6 and E7 genes of HPV were sequenced and compared with the HPV reference for sequence analysis. Phylogenetic trees were constructed using the neighbour-joining tree method with MEGA 7.0.ResultsIn this study, 233 E6 and 212 E7 sequences were successfully amplified by PCR, and these sequences were divided into 5 species groups: alpha-9 (HPV16, 31, 33, 52, 58), alpha-5 (HPV51), alpha-6 (HPV53, 66), alpha-7 (HPV18, 39, 59, 68) and alpha-10 (HPV6, 44). The incidence of high-grade squamous intraepithelial lesion (HSIL) in patients infected with alpha-9 HPV was significantly increased compared with other groups (P < 0.0001), especially HPV16 (P < 0.0001). Strikingly, E7 had significantly fewer nonsynonymous variants in the HSIL compared to

中文翻译：

人乳头瘤病毒 E6 和 E7 的系统发育和多态性：alpha-9 (HPV16, 31, 33, 52, 58), alpha-5 (HPV51), alpha-6 (HPV53, 66), alpha-7 (HPV18, 39) , 59, 68) 和 alpha-10 (HPV6, 44) 来自上海的女性

背景人乳头瘤病毒（HPVs）的持续感染与宫颈上皮内瘤变（CIN）和宫颈癌有关。然而，为什么只有一小部分HPV病例进展为癌症仍不清楚。方法我们关注262例HPV阳性宫颈病变患者14种常见HPV类型变异的异质性、分类、进化和扩散。对 HPV 的 E6 和 E7 基因进行测序，并与 HPV 参考进行序列分析比较。采用MEGA 7.0，采用neighbour-joining tree方法构建系统发育树。结果本研究通过PCR成功扩增出233个E6和212个E7序列，将这些序列分为5个种群：α-9（HPV16、31、33） , 52, 58), alpha-5 (HPV51), alpha-6 (HPV53, 66), alpha-7 (HPV18, 39, 59, 68) 和 alpha-10 (HPV6, 44)。与其他组相比，感染 alpha-9 HPV 的患者高级别鳞状上皮内病变 (HSIL) 的发生率显着增加 (P < 0.0001)，尤其是 HPV16 (P < 0.0001)。引人注目的是，与 E7 相比，E7 在 HSIL 中的非同义变体明显更少

更新日期：2019-11-21

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南

全部期刊列表>>