The ascomycete fungus, Pyricularia oryzae or Magnaporthe oryzae, is known to cause blast disease in more than 80 host plants of the Gramineae family—cereals including rice and grasses. The improvement of the Supa234 rice line (IR97012-27-3-1-1-B, containing badh2 gene for aroma) developed at IRRI-ESA Burundi consisted of introgression of R genes (Pita and Pi9) for blast resistance. The F₂ population obtained via the cross had been screened for blast resistance using inoculation with Pyricularia oryzae spore’s suspension. The objectives of this study were to assess the presence of Pita and Pi9 genes for blast resistance and to assess the presence of the badh2 gene for aroma in the screened F₂ plants using molecular markers. Genotyping was carried out in 103 F₂ plants which grew to maturity using the KASP genotyping method with SNP markers (snpOS0007, snpOS0006, and snpOS0022) targeting the Pita and Pi9 genes for blast resistance and the badh2 gene for aromatic fragrance. The genotyping results showed that 38 F₂ plants had the Pita gene present in both alleles, 31 F₂ plants with the Pita gene in one allele, and only one plant (3B1) was found with the Pi9 gene in one allele. The badh2 gene for aroma was detected in 27 F₂ plants on both alleles and in 57 F₂ plants on one allele. There were thirteen plants which had both the Pita gene and the badh2 gene for aroma, and only one plant (3B1) had a combination of the three genes (Pita, Pi9, and badh2). Seven plants resistant to blast disease (2H2, 2H4, 1G2, 1C12, 1E13, 1B12, and 1C5) with the Pita and badh2 genes were found, and only one resistant plant (3B1) had a combination of the three genes Pi9, Pita, and badh2 which is recommended to be bulked for the development of the Supa aromatic rice variety resistant to blast disease. The plants generated by the best line 3B1 should further be evaluated for grain quality (Supa type) after F₅ generation in the field.

中文翻译：

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Supa芳香水稻（Oryza sativa L.）F2种群抗稻瘟病（Pyricularia oryzae）基因的基因分型。

众所周知，子囊菌，稻瘟病菌或稻瘟病菌会在禾本科科的80多种寄主植物中引起稻瘟病，谷物包括稻米和草。在IRRI-ESA布隆迪开发的Supa234水稻品系（IR97012-27-3-1-1-B，包含badh2基因用于芳香）的改良包括R基因（Pita和Pi9）的基因渗入，以提高抗稻瘟病性。通过接种稻瘟病菌孢子悬浮液，筛选了通过杂交获得的F ₂群体的抗瘟性。这项研究的目的是评估Pita和Pi9的存在基因用于抗稻瘟病，并使用分子标记评估在筛选的F ₂植物中存在的badh2基因是否具有香气。基因型分型是在103株F ₂植物中进行的，该植物使用KASP基因分型方法成熟至成熟，该植物带有SNP标记（snpOS0007，snpOS0006和snpOS0022），其针对Pita和Pi9基因具有抗爆炸性，而badh2基因具有芳香性。基因分型结果表明，38种女性_2种植物具有皮塔基因存在于两个等位基因，31种女性_2种植物与皮塔基因在一个等位基因，并且只有一株植物（3B1）被发现与聚合Pi9一个等位基因中的基因。所述BADH2在27种F的检测到香气基因_2种在两个等位基因上的植物和在57种女性_2种上一个等位基因的植物。有13个植物同时具有Pita基因和badh2基因的香气，只有一种植物（3B1）具有这三个基因（Pita，Pi9和badh2）的组合。发现了七株对带有Pita和badh2基因的瘟病（2H2、2H4、1G2、1C12、1E13、1B12和1C5）产生抗性的植物，而只有一株抗性植物（3B1）具有三个基因Pi9，Pita和和badh2建议将其大量用于开发抗稻瘟病的Supa香米品种。最好的3B1系产生的植物在田间F ₅产生后应进一步评估谷物品质（Supa型）。