Spheroid culture is a widely used three-dimensional culture technology that simulates the three-dimensional structure of tumors in vivo and has been considered a good model for tumor research. However, current commercialized spheroid culture tools have the shortcomings of high cost or relatively poor spheroid-forming results for some special cells. To solve such problems, we designed a 3D printed, reusable, stamp-like resin mold that could shape microstructures for spheroid culture of tumor cells on the surface of agarose substrate in a 96-well plate. We applied this homemade three-dimensional culture tool in spheroid formation for hepatocellular carcinoma cells. The experimental data show that the effect of spheroid culture on four hepatocellular carcinoma cell lines in our homemade spheroid culture plate is better than that of the commercialized ultralow attachment spheroid culture plate, and compared to two-dimensional culture, three-dimensional culture improves cell functions. In addition, the drug-sensitive test based on patient-derived hepatocellular carcinoma cells showed a different pattern between spheroid and two-dimensional cultures. In conclusion, our spheroid culture tool is characterized by its low cost, reusability, low cell consumption, convenience in medium exchange, and good effect of spheroid formation, suggesting that this technique could be widely used in individual treatment and high-throughput drug screening.

中文翻译：

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使用新型邮票样工具的高通量三维球形肿瘤模型。

球体培养是一种广泛使用的三维培养技术，可模拟体内肿瘤的三维结构，被认为是肿瘤研究的良好模型。然而，当前商业化的球状体培养工具对于某些特殊细胞具有成本高或球状体形成结果相对较差的缺点。为了解决这些问题，我们设计了3D打印的，可重复使用的，像邮票一样的树脂模具，该模具可以在96孔板的琼脂糖底物表面上形成用于肿瘤细胞球形培养的微结构。我们将这种自制的三维培养工具应用于肝癌细胞的球体形成。实验数据表明，在我们自制的球状培养板中，球状培养对四种肝癌细胞系的影响要优于商品化的超低附着球状培养板，并且与二维培养相比，三维培养可改善细胞功能。另外，基于患者来源的肝癌细胞的药物敏感性试验显示出球形和二维培养物之间存在不同的模式。综上所述，我们的球形培养工具具有成本低，可重复使用，细胞消耗低，培养基更换方便，球形形成效果好等特点，表明该技术可广泛用于个体化治疗和高通量药物筛选。与二维培养相比，三维培养可改善细胞功能。另外，基于患者来源的肝癌细胞的药物敏感性试验显示出球形和二维培养物之间存在不同的模式。综上所述，我们的球形培养工具具有成本低，可重复使用，细胞消耗低，培养基更换方便，球形形成效果好等特点，表明该技术可广泛用于个体化治疗和高通量药物筛选。与二维培养相比，三维培养可改善细胞功能。另外，基于患者来源的肝癌细胞的药物敏感性试验显示出球形和二维培养物之间存在不同的模式。综上所述，我们的球形培养工具具有成本低，可重复使用，细胞消耗低，培养基更换方便，球形形成效果好等特点，表明该技术可广泛用于个体化治疗和高通量药物筛选。