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Super-antibiofilm effect of N2 plasma treated buffer (NPB) against plant pathogenic bacterium.
Journal of Biological Engineering ( IF 5.6 ) Pub Date : 2019-12-04 , DOI: 10.1186/s13036-019-0222-z
Hyemi Seo 1, 2 , Jisoo Hong 1, 3 , Taeyeol Kim 1 , Won Ll Choi 2 , Daekyung Sung 2 , Eunpyo Moon 1
Affiliation  

Controlling of biofilms formation in numerous pathogenic bacteria is one of the most difficult tasks in the control of bacterial diseases. Plasma has attracted extensive attention due to their potential applications for effective inhibiting of biofilm. Recently, plasma-activated water (PAW) has developed as an alternative method for bacterial inactivation and disinfection of foods owing to advantages of more convenient and efficient storage and transportation than direct plasma application. However, most previous studies about PAW have only focused on the improvement of its antibacterial effect instead of antibiofilm activity. Therefore, we report the development of N2 plasma treated buffer (NPB) and the super-antibiofilm effect of NPB against Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) as a plant pathogenic bacterium. Scavenger assays using various antioxidants revealed that reactive oxygen species were involved in the inhibitory cellular actions of NPB, with H2O2 and singlet oxygen proving essential for bacterial death. Intensive analysis of NPB, stored at different periods and temperatures, showed that the antimicrobial efficacy was well maintained for 3 months at - 80 °C. Importantly, further studies showed that NPB effectively inhibited not only the growth of planktonic Pst DC3000 but also biofilm formation. The remarkable inhibition on the biofilm was analyzed and visualized using LIVE/DEAD viability assays and confocal laser scanning microscopy (CLSM) imaging. The 3D CLSM imaging data revealed that the bactericidal activity of NPB was permeable enough to affect the cells embedded inside the biofilm. This prominent permeability could be a crucial feature of NPB contributing to effective super-antibiofilm.

中文翻译:

N2血浆处理过的缓冲液(NPB)对植物病原细菌的超强抗生物膜作用。

控制许多病原细菌中生物膜的形成是控制细菌疾病中最困难的任务之一。血浆由于其有效抑制生物膜的潜在应用而受到广泛关注。近来,由于比直接施加等离子体更方便和有效的储存和运输的优点,等离子体活化水(PAW)已经发展为食品的细菌灭活和消毒的替代方法。但是,以前有关PAW的大多数研究仅集中于其抗菌作用的改善,而不是抗生物膜的活性。因此,我们报道了N2血浆处理的缓冲液(NPB)的发展和NPB对丁香假单胞菌PV的超强抗生物膜作用。番茄DC3000(Pst DC3000)作为植物病原细菌。使用各种抗氧化剂的清除剂分析表明,活性氧参与了NPB的抑制性细胞作用,其中过氧化氢和单线态氧被证明对细菌死亡至关重要。对在不同时期和温度下储存的NPB进行的深入分析表明,其抗菌功效在-80°C下可保持3个月。重要的是,进一步的研究表明,NPB不仅有效抑制了浮游性Pst DC3000的生长,而且还抑制了生物膜的形成。使用LIVE / DEAD活力测定和共聚焦激光扫描显微镜(CLSM)成像分析并可视化了对生物膜的显着抑制作用。3D CLSM成像数据表明,NPB的杀菌活性具有足够的渗透性,可影响生物膜内部嵌入的细胞。
更新日期:2020-04-22
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