To investigate the stability and dynamic characteristics of monolayer adsorbed on unsaturated lipid dioleoylphosphatidylcholine (DOPC) with varying concentrations of myelin basic protein (MBP), the system is studied by applying Langmuir technique and making atomic force microscope (AFM) observation, which is based on the mass conservation equation analysis method referred to in the thermodynamics theory. As indicated by surface pressure-mean molecular area (π − A) and surface pressure-adsorption time (π − T) isotherms, the physical properties of monolayer derived from the interaction of varying concentrations of MBP with liquid crystalline unsaturated lipid DOPC molecules were qualitatively studied. As revealed by surface morphology analysis with AFM, the micro region was expanded as the concentration of MBP in the subphase was on the increase, suggesting that hydrophobic interactions led to the MBP insertion, thus causing accumulation of the MBP on the surface of the monolayer. Experimental results have demonstrated that the partition coefficient of the interaction between MBP and unsaturated phospholipid DOPC and the molecular area of MBP adsorbed on the monolayer film was calculated using the mass conservation equation. In addition, not only does the varying concentration of MBP in the subphase exerts significant effects on the arrangement and conformation of DOPC monolayer, it also has certain guiding significance to exploring the structural changes to biofilm supramolecular aggregates as well as the pathogenesis and treatment of related diseases.

中文翻译：

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液晶二油酰磷脂酰胆碱单层吸附髓鞘碱性蛋白的热力学分析

为了研究不同浓度髓鞘碱性蛋白（MBP）吸附在不饱和脂质二油酰磷脂酰胆碱（DOPC）上的单分子层的稳定性和动力学特性，应用朗缪尔技术和原子力显微镜（AFM）观察研究该系统，该系统基于热力学理论中所指的质量守恒方程分析方法。如表面压力-平均分子面积（π-A）和表面压力-吸附时间（π-T）等温线所示，不同浓度的MBP与液晶不饱和脂质DOPC分子相互作用产生的单层物理性质定性学习了。AFM 表面形态分析表明，随着亚相中 MBP 浓度的增加，微观区域扩大，表明疏水相互作用导致 MBP 插入，从而导致 MBP 在单层表面积累。实验结果表明，MBP与不饱和磷脂DOPC相互作用的分配系数和吸附在单层膜上的MBP的分子面积可用质量守恒方程计算。此外，亚相中不同浓度的MBP不仅对DOPC单层的排列和构象产生显着影响，对探索生物膜超分子聚集体的结构变化以及相关疾病的发病机制和治疗也具有一定的指导意义。疾病。实验结果表明，MBP与不饱和磷脂DOPC相互作用的分配系数和吸附在单层膜上的MBP的分子面积可用质量守恒方程计算。此外，亚相中不同浓度的MBP不仅对DOPC单层的排列和构象产生显着影响，对探索生物膜超分子聚集体的结构变化以及相关疾病的发病机制和治疗也具有一定的指导意义。疾病。实验结果表明，MBP与不饱和磷脂DOPC相互作用的分配系数和吸附在单层膜上的MBP的分子面积可用质量守恒方程计算。此外，亚相中不同浓度的MBP不仅对DOPC单层的排列和构象产生显着影响，对探索生物膜超分子聚集体的结构变化以及相关疾病的发病机制和治疗也具有一定的指导意义。疾病。