Hydrolysed proteins from sunflower seed and peanut were separated by ultrafiltration. Results showed that 1–3 kDa sunflower seed peptide (SSP₄) and ≥10 kDa peanut peptide (PP₁) fractions had the highest Ca‐binding capacity of 104.80 and 124.74 mg g⁻¹, respectively. After Ca binding, scanning electron microscopy results showed that the loose sheet‐like structure of SSP₄ and PP₁ transformed into a granular structure. Fourier‐transform infrared spectroscopy results showed that carboxyl and amide groups were involved in Ca binding. Growing male mice were assigned to eight groups: control, CaCO₃ and six experimental groups (low, medium and high doses for each SSP₄‐Ca and PP₁‐Ca). Apparent Ca absorption rate, bone mineral density (BMD), bone surface microstructure and biomechanical index and serum biochemical index were evaluated. Animal experiment results indicated that SSP₄‐Ca and PP₁‐Ca can improve Ca bioavailability; effectively enhance BMD and structure; and promote Ca absorption. The peptide–Ca complexes were absorbed better than CaCO₃ and promoted Ca accumulation in the bone. PP₁‐Ca was better than SSP₄‐Ca in promoting calcium absorption and enhancing BMD.

中文翻译：

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葵花籽和花生中肽钙复合物对增强骨矿物质密度的影响

通过超滤分离向日葵种子和花生中的水解蛋白。结果表明，1–3 kDa葵花籽肽（SSP ₄）和≥10kDa花生肽（PP ₁）馏分具有最高的Ca结合能力，分别为104.80和124.74 mg g ^-1。Ca结合后，扫描电子显微镜结果显示SSP ₄和PP ₁的松散片状结构转变为颗粒状结构。傅立叶变换红外光谱结果表明，羧基和酰胺基团参与Ca的结合。将生长中的雄性小鼠分为八组：对照组，CaCO ₃和六个实验组（每种SSP _4的低，中和高剂量）-Ca和PP ₁ -Ca）。评价表观钙吸收率，骨矿物质密度（BMD），骨表面微观结构，生物力学指标和血清生化指标。动物实验结果表明，SSP ₄ -Ca和PP ₁ -Ca可以提高Ca的生物利用度。有效增强骨密度和结构；并促进钙的吸收。肽-Ca复合物比CaCO ₃吸收更好，并促进了Ca在骨骼中的积累。PP ₁ ‐Ca在促进钙吸收和增强BMD方面优于SSP ₄ ‐Ca。