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Nebulized jet-based printing of bio-electrical scaffolds for neural tissue engineering: a feasibility study.
Biofabrication ( IF 9 ) Pub Date : 2020-01-30 , DOI: 10.1088/1758-5090/ab71e0
Miriam Seiti 1 , Paola Ginestra , Rosalba Monica Ferraro , Elisabetta Ceretti , Eleonora Ferraris
Affiliation  

In this paper, we investigate the application of a direct writing technique to print conductive patterns onto a bio-compatible electrospun-pyrolysed carbon fibre-based substrate. The result is a first study towards the production of bio-electrical scaffolds that could be used to enhance the promotion of efficient connections among neurons for in vitro studies in the widespread field of neural tissue engineering. An electrospinning process is employed for the production of the materials derived from the precursor Polyacrylonitrile (PAN), in which the analysis of the embedment of carbon nanotubes (CNTs) is also investigated. Subsequently, the research methodology of suitable printed electronic parameters, using a commercial silver silver nano-particle (AgNPs, Øavg,particle size ~ 100 nm) ink, is described. The results show values of 2 Ω*cm in the resistivity of the carbon-fibre materials and conductive printed lines of ~50 Ω resistance on glass, and less than ~140 Ω on carbon-fibre samples. Biocompatibility results demonstrate the possibility to use electrospun-pyrolysed mats, also with the embedment of CNTs, as potential neural substrates for a spatially localized electrical stimulation (ES) across a tissue. In addition, the data concerning the potential toxicity of silver suspension are in accordance with the literature, showing a dose-dependent behaviour. This work is pioneer in the feasibility study of using the flexible and versatile printed electronic (PE) approach, combined with engineered bio-compatible substrates, for the realisation of integrated bio-electrical scaffolds for in vitro neural tissue engineering applications.

中文翻译:

基于雾化的基于喷射的生物组织神经细胞工程电动支架印刷:可行性研究。

在本文中,我们研究了直接书写技术在将导电图案印刷到生物相容的电纺热解碳纤维基基材上的应用。该结果是对生产生物电支架的第一项研究,该支架可用于增强神经元之间有效连接的促进,以用于神经组织工程广泛领域中的体外研究。采用静电纺丝工艺生产衍生自前体聚丙烯腈(PAN)的材料,其中还研究了碳纳米管(CNT)的嵌入分析。随后,描述了使用商业化的银纳米银(AgNPs,Øavg,粒径约100 nm)油墨对合适的印刷电子参数进行研究的方法。结果表明,碳纤维材料和导电印刷线路的电阻率值为2Ω* cm,在玻璃上约为50Ω,而在碳纤维样品上约为140Ω。生物相容性结果表明,也可以将电纺热解垫(也嵌入CNT)用作组织中空间局部电刺激(ES)的潜在神经基质。此外,有关银悬浮液潜在毒性的数据与文献一致,显示出剂量依赖性的行为。这项工作是将柔性和多功能印刷电子(PE)方法与工程化的生物相容性基材结合使用以实现用于体外神经组织工程应用的集成生物电支架的可行性研究的先驱。
更新日期:2020-03-04
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