Arsenic (As) is a toxic element that is highly abundant in the environment. However, there has not been sufficient research into the mechanisms of arsenic-induced transgenerational effects. In biomedical and environmental toxicology research field, C. elegans are often used as the ideal model. In this study, F0 generation animals were cultured with arsenite, while subsequent generations animals (F1 - F6) were cultured in the absence of arsenic. Experiments were performed to examine the transgenerational glycometabolism and the associated mechanisms in all seven generations (F0 - F6) of C. elegans. Results show that arsenite exposure increased total glucose content but reduced glucose metabolites in F0 generation C. elegans. The total glucose content was also elevated in subsequent generations probably due to transgenerational downregulation of fgt-1. In addition, arsenite exposure induced transgenerational downregulation of histone demethyltransferase spr-5 and elevated histone dimethylation in F0 generation. This study highlights that single generation exposure to arsenite causes transgenerational changes in glycometabolism in C. elegans, which may be caused by downregulation of spr-5 and elevation of H3K4me2.

中文翻译：

---

亚砷酸盐诱导的跨代糖代谢通过抑制秀丽隐杆线虫中的 spr-5 与 H3K4me2 的上调相关

砷 (As) 是一种有毒元素，在环境中含量很高。然而，对砷诱导的跨代效应的机制还没有足够的研究。在生物医学和环境毒理学研究领域，秀丽隐杆线虫常被用作理想模型。在本研究中，F0 代动物与亚砷酸盐一起培养，而后代动物（F1 - F6）则在没有砷的情况下培养。进行了实验以检查所有七代 (F0 - F6) 线虫的跨代糖代谢和相关机制。结果表明，亚砷酸盐暴露增加了 F0 代秀丽隐杆线虫的总葡萄糖含量，但减少了葡萄糖代谢物。可能由于 fgt-1 的跨代下调，后代的总葡萄糖含量也升高。此外，亚砷酸盐暴露诱导组蛋白去甲基转移酶 spr-5 的跨代下调和 F0 代组蛋白二甲基化升高。该研究强调，单代亚砷酸盐暴露导致秀丽隐杆线虫糖代谢的跨代变化，这可能是由 spr-5 的下调和 H3K4me2 的升高引起的。