Staphylococcus aureus are known to cause diseases from normal skin wound to life intimidating infections. Among the drug resistant strain, management of methicillin resistant Staphylococcus aureus (MRSA) is very difficult by using conventional antibiotic treatment. Both Zinc oxide nanoparticles (ZnONPs) and pancreatin (PK) are known to have antibacterial activity. Our main objective is to dope PK on ZnONPs to reduced zinc-oxide toxicity but increased anti-bacterial and anti-biofilms activity. In present study, we showed that, functions of zinc oxide nanoparticles with pancreatin enzyme (ZnONPs-PK) have anti-bacterial, anti-biofilms, anti-motility and anti-virulence properties against MRSA. Moreover, ZnONPs-PK were more potent to eradicate MRSA than only ZnONPs and PK. Application of the produced nano-composites as treatment on infected swine dermis predominantly reflects the potential treatment property of it. The vancomycin sensitivity of MRSA was significantly increased on application with ZnONPs-PK. Further study revealed cell membrane was the target of the ZnONPs-PK and that leads to oxidative damage of the cells. The produced nanoparticles were found completely non-toxic to human's keratinocytes and lung epithelial cell lines at its bactericidal concentration. Overall, this study emphasizes the potential mechanisms underlying the selective bactericidal properties of ZnONPs-PK against MRSA. This novel nanoparticle strategy may provide the ideal solution for comprehensive management of MRSA and its associated diseases with minimising the use of antibiotics.

中文翻译：

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胰酶掺杂的氧化锌纳米颗粒对耐甲氧西林的金黄色葡萄球菌的抗菌，抗生物膜活性和作用机理。

已知金黄色葡萄球菌会引起从正常皮肤伤口到威胁生命的感染的疾病。在耐药菌株中，使用常规抗生素治疗很难控制耐甲氧西林金黄色葡萄球菌（MRSA）。已知氧化锌纳米颗粒（ZnONPs）和胰酶（PK）均具有抗菌活性。我们的主要目标是在ZnONP上掺杂PK，以减少氧化锌的毒性，但增加抗菌和抗生物膜的活性。在本研究中，我们表明，具有胰酶的纳米氧化锌（ZnONPs-PK）的功能具有抗MRSA的抗菌，抗生物膜，抗运动性和抗毒力特性。此外，ZnONPs-PK比仅ZnONPs和PK更能消除MRSA。产生的纳米复合材料作为感染猪真皮的治疗方法的应用主要反映了其潜在的治疗特性。使用ZnONPs-PK时，MRSA的万古霉素敏感性显着提高。进一步的研究表明，细胞膜是ZnONPs-PK的靶标，并导致细胞的氧化损伤。发现所产生的纳米颗粒在其杀菌浓度下对人的角质形成细胞和肺上皮细胞系完全无毒。总的来说，这项研究强调了ZnONPs-PK对MRSA的选择性杀菌特性的潜在机制。这种新颖的纳米颗粒策略可以为MRSA及其相关疾病的综合管理提供理想的解决方案，同时减少抗生素的使用。