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Oleogel-mediated transdermal delivery of white emitting NaYF4 conjugated with Rose Bengal for the generation of reactive oxygen species through NIR-upconversion.
Colloids and Surfaces B: Biointerfaces ( IF 5.8 ) Pub Date : 2020-03-04 , DOI: 10.1016/j.colsurfb.2020.110945
Soumyashree Dhal 1 , Preeti Verma 1 , Monalisa Mishra 2 , Supratim Giri 1
Affiliation  

The transdermal route for the delivery of therapeutic agents to the inner skin tissues for non-invasive photodynamic therapy; though constitutes a desired modality for treating skin cancer, the success has been limited due to the insurmountable nature of the stratum corneum (SC). In this context, for the first time we report the localization of photosensitizer-conjugated upconversion (UC) particles to the deeper dermal region by overcoming SC through an oleogel-mediated transport mechanism for NIR-induced photodynamic production of reactive oxygen species (ROS). We developed soybean oil and stearic acid based oleogels by incorporating photoluminescent white light emitting NaYF4 (WEN) upconversion (UC) particles conjugated with Rose Bengal (RB), termed as WEN-RB-G. Similarly, we fabricated another type of oleogel by incorporating Li+ doped WEN based UC particles (RB conjugated), with 10 times more photoluminescence intensity, termed as LiWEN-RB-G. Based on the skin permeation enhancing effect of the constituents of the oleogels, we demonstrated the permeation of these two types of conjugated particles in microgram scale through the full thickness of the pig ear skin model within 48 h. The localization of the conjugated particles throughout the skin tissue including dermal and epidermal region was confirmed by confocal microscopy. We also conducted a comparative assessment on WEN-RB-G and LiWEN-RB-G for the suitability of ROS generation and bioimaging under NIR activation. The 'proof of principle' concept reported here is expected to frame a gateway in future for NIR-induced photo-theranostics targeting skin cancer.

中文翻译:

油凝胶介导的白色发射NaYF4与玫瑰红偶联的透皮递送,可通过近红外转换产生活性氧。

用于将治疗剂递送至皮肤内组织以进行非侵入性光动力疗法的透皮途径;尽管构成治疗皮肤癌的理想方式,但由于角质层(SC)的不可克服的性质,其成功受到了限制。在这种情况下,我们首次报道了通过油凝胶介导的转运机制克服SC的光敏剂共轭上转换(UC)粒子定位到较深层的真皮区域,从而通过NIR诱导的活性氧(ROS)的光动力产生。我们通过掺入与玫瑰红(RB)共轭的光致发白光的NaYF4(WEN)上转换(UC)粒子(称为WEN-RB-G),开发了基于大豆油和硬脂酸的油凝胶。同样,我们通过掺入Li +掺杂的WEN基UC颗粒(RB共轭)制备了另一种类型的油凝胶,其光致发光强度提高了10倍,称为LiWEN-RB-G。基于油精成分的皮肤渗透增强作用,我们证明了这两种类型的共轭颗粒在48小时内以微克级的渗透率穿过了猪耳皮肤模型的整个厚度。共聚焦显微镜证实了共轭颗粒在包括皮肤和表皮区域在内的整个皮肤组织中的定位。我们还对WEN-RB-G和LiWEN-RB-G进行了近红外激活下ROS生成和生物成像的适用性比较评估。“原则证明”
更新日期:2020-03-04
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