Ole e 7 allergen from Olea europaea pollen possesses a major clinical relevance because it produces severe symptoms, such as anaphylaxis, in allergic patients exposed to high olive pollen counts. Ole e 7 is a non-specific lipid transfer protein (nsLTP) characterized by the presence of a tunnel-like hydrophobic cavity, which may be suitable for hosting and, thus, transporting lipids -as it has been described for other nsLTPs-. The identification of the primary amino acid sequence of Ole e 7, and its production as a recombinant allergen, allowed characterizing its lipid-binding properties and its effect at air-liquid interfaces. Fluorescence and interferometry experiments were performed using different phospholipid molecular species and free fatty acids to analyse the lipid-binding ability and specificity of the allergen. Molecular modelling of the allergen was used to determine the potential regions involved in lipid interaction. Changes in Ole e 7 structure after lipid interaction were analysed by circular dichroism. Changes in the IgE binding upon ligand interaction were determined by ELISA. Wilhelmy balance measurements and fluorescence surfactant adsorption tests were performed to analyse the surface activity of the allergen. Using these different approaches, we have demonstrated the ability of Ole e 7 to interact and bind to a wide range of lipids, especially negatively charged phospholipids and oleic acid. We have also identified the protein structural regions and the residues potentially involved in that interaction, suggesting how lipid-protein interactions could define the behaviour of the allergen once inhaled at the airways.

来自油橄榄的Ole e 7过敏原花粉具有重要的临床意义，因为在暴露于高橄榄花粉量的过敏患者中，花粉会产生严重的症状，例如过敏反应。Ole e 7是一种非特异性脂质转移蛋白（nsLTP），其特征在于存在隧道状疏水腔，该通道可能适合于容纳脂质并因此转运脂质（如针对其他nsLTP所述）。Ole e 7的一级氨基酸序列的鉴定以及其作为重组变应原的生产，可以表征其脂质结合特性及其对气液界面的影响。使用不同的磷脂分子种类和游离脂肪酸进行荧光和干涉测量实验，以分析变应原的脂质结合能力和特异性。过敏原的分子模型被用来确定参与脂质相互作用的潜在区域。通过圆二色性分析脂质相互作用后Ole e 7结构的变化。通过ELISA确定在配体相互作用时IgE结合的变化。进行了威廉的平衡测量和荧光表面活性剂吸附测试，以分析过敏原的表面活性。使用这些不同的方法，我们证明了Ole e 7与多种脂质（尤其是带负电荷的磷脂和油酸）相互作用并结合的能力。我们还确定了相互作用中可能涉及的蛋白质结构区域和残基，这表明脂质-蛋白质相互作用一旦被气道吸入，将如何定义过敏原的行为。