当前位置: X-MOL 学术J. Pathol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Applying single-cell technologies to clinical pathology: progress in nephropathology.
The Journal of Pathology ( IF 7.3 ) Pub Date : 2020-03-31 , DOI: 10.1002/path.5417
Benjamin J Stewart 1, 2, 3 , Menna R Clatworthy 1, 2, 3
Affiliation  

Cells represent the basic building blocks of living organisms. Accurate characterisation of cellular phenotype, intercellular signalling networks, and the spatial organisation of cells within organs is crucial to deliver a better understanding of the processes underpinning physiology, and the perturbations that lead to disease. Single-cell methodologies have increased rapidly in scale and scope in recent years and are set to generate important insights into human disease. Here, we review current practices in nephropathology, which are dominated by relatively simple morphological descriptions of tissue biopsies based on their appearance using light microscopy. Bulk transcriptomics have more recently been used to explore glomerular and tubulointerstitial kidney disease, renal cancer, and the responses to injury and alloimmunity in kidney transplantation, generating novel disease insights and prognostic biomarkers. These studies set the stage for single-cell transcriptomic approaches that reveal cell-type-specific gene expression patterns in health and disease. These technologies allow genome-wide disease susceptibility genes to be interpreted with the knowledge of the specific cell populations within organs that express them, identifying candidate cell types for further study. Single-cell technologies are also moving beyond assaying individual cellular transcriptomes, to measuring the epigenetic landscape of single cells. Single-cell antigen-receptor gene sequencing also enables specific T- and B-cell clones to be tracked in different tissues and disease states. In the coming years these rich 'multi-omic' descriptions of kidney disease will enable histopathological descriptions to be comprehensively integrated with molecular phenotypes, enabling better disease classification and prognostication and the application of personalised treatment strategies. © 2020 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of Pathological Society of Great Britain and Ireland.

中文翻译:

将单细胞技术应用于临床病理学:肾病理学进展。

细胞代表生物体的基本组成部分。细胞表型、细胞间信号网络和器官内细胞的空间组织的准确表征对于更好地理解支撑生理学的过程以及导致疾病的扰动至关重要。近年来,单细胞方法学的规模和范围迅速扩大,并将对人类疾病产生重要的见解。在这里,我们回顾了肾脏病理学的当前实践,这些实践主要是使用光学显微镜根据组织活检的外观对组织活检进行相对简单的形态学描述。最近,大量转录组学被用于探索肾小球和肾小管间质性肾病、肾癌以及肾移植中对损伤和同种免疫的反应,产生新的疾病见解和预后生物标志物。这些研究为单细胞转录组学方法奠定了基础,揭示了健康和疾病中细胞类型特异性的基因表达模式。这些技术允许利用表达这些基因的器官内特定细胞群的知识来解释全基因组疾病易感性基因,从而确定候选细胞类型以供进一步研究。单细胞技术也超越了分析单个细胞转录组,转向测量单细胞的表观遗传景观。单细胞抗原受体基因测序还可以追踪不同组织和疾病状态下的特定 T 细胞和 B 细胞克隆。在未来几年中,这些丰富的肾脏疾病“多组学”描述将使组织病理学描述与分子表型全面整合,从而实现更好的疾病分类和预后以及个性化治疗策略的应用。© 2020 作者。《病理学杂志》由 John Wiley & Sons Ltd 代表大不列颠及爱尔兰病理学会出版。
更新日期:2020-04-22
down
wechat
bug