Design and development of highly bioactive nanoscale biomaterials with enhanced osteogenic differentiation on adipose stem cells is rather important for bone regeneration and attracting much attention. Herein, monodispersed glycerophosphate‐decorated bioactive glass nanoparticles (BGN@GP) are designed and their effect is investigated on the osteogenic differentiation of adipose mesenchymal stem cells (ADMSCs) and in vivo bone regeneration. The surface‐modified BGN@GP can be efficiently taken by ADMSCs and shows negligible cytotoxicity. The in vitro results reveal that BGN@GP significantly enhances the alkaline phosphatase activity and calcium biominerialization of ADMSCs either under normal or osteoinductive medium as compared to BGNs. Further studies find that the osteogenic genes and proteins including Runx2 and Bsp in ADMSCs are significantly improved by BGN@GP even under normal culture medium. The in vivo animal experiment confirms that BGN@GP significantly promotes the new bone formation in a rat skull defect model. This study suggests that bioactive small molecule decorating is an efficient strategy to improve the osteogenesis capacity of inorganic ceramics nanomaterials.

中文翻译：

---

单分散的β-甘油磷酸修饰的生物活性玻璃纳米颗粒增强脂肪干细胞的成骨分化能力和体内骨再生

具有增强的脂肪干细胞成骨分化能力的高生物活性纳米级生物材料的设计和开发对于骨骼再生非常重要，并引起了广泛关注。本文设计了单分散甘油磷酸修饰的生物活性玻璃纳米颗粒（BGN @ GP），并研究了它们对脂肪间充质干细胞（ADMSCs）的成骨分化和体内骨骼再生的影响。经过表面修饰的BGN @ GP可以被ADMSC有效吸收，并且显示出可忽略的细胞毒性。体外结果显示，与BGNs相比，在正常或骨诱导培养基下，BGN @ GP均可显着增强ADMSCs的碱性磷酸酶活性和钙生物矿化作用。进一步的研究发现，即使在正常培养基下，BGN @ GP也会显着改善ADMSCs中的成骨基因和蛋白（包括Runx2和Bsp）。体内动物实验证实BGN @ GP在大鼠颅骨缺损模型中显着促进了新的骨形成。这项研究表明，生物活性小分子装饰是提高无机陶瓷纳米材料成骨能力的有效策略。