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Multiplexed mass spectrometry of individual ions improves measurement of proteoforms and their complexes
Nature Methods ( IF 48.0 ) Pub Date : 2020-03-02 , DOI: 10.1038/s41592-020-0764-5 Jared O Kafader 1 , Rafael D Melani 1 , Kenneth R Durbin 1 , Bon Ikwuagwu 2 , Bryan P Early 1 , Ryan T Fellers 1 , Steven C Beu 3 , Vlad Zabrouskov 4 , Alexander A Makarov 5 , Joshua T Maze 6 , Deven L Shinholt 6 , Ping F Yip 4 , Danielle Tullman-Ercek 2 , Michael W Senko 4 , Philip D Compton 1 , Neil L Kelleher 1
中文翻译：
单个离子的多路复用质谱法改进了蛋白质型及其复合物的测量
更新日期：2020-03-02
Nature Methods ( IF 48.0 ) Pub Date : 2020-03-02 , DOI: 10.1038/s41592-020-0764-5 Jared O Kafader 1 , Rafael D Melani 1 , Kenneth R Durbin 1 , Bon Ikwuagwu 2 , Bryan P Early 1 , Ryan T Fellers 1 , Steven C Beu 3 , Vlad Zabrouskov 4 , Alexander A Makarov 5 , Joshua T Maze 6 , Deven L Shinholt 6 , Ping F Yip 4 , Danielle Tullman-Ercek 2 , Michael W Senko 4 , Philip D Compton 1 , Neil L Kelleher 1
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An Orbitrap-based ion analysis procedure determines the direct charge for numerous individual protein ions to generate true mass spectra. This individual ion mass spectrometry (I2MS) method for charge detection enables the characterization of highly complicated mixtures of proteoforms and their complexes in both denatured and native modes of operation, revealing information not obtainable by typical measurements of ensembles of ions.
中文翻译：
单个离子的多路复用质谱法改进了蛋白质型及其复合物的测量
基于 Orbitrap 的离子分析程序可确定大量单个蛋白质离子的直接电荷，从而生成真正的质谱。这种用于电荷检测的单个离子质谱 (I 2 MS) 方法能够在变性和天然操作模式下表征高度复杂的蛋白质及其复合物混合物，揭示通过离子集合的典型测量无法获得的信息。
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