A label-free fluorescent nanoprobe for iodide ion (I-) detection was developed based on the direct fluorescence quenching of spermine-functionalized carbon dots (SC-dots), whether in complex biological fluids or living cells. The positively charged SC-dots were fabricated via one-step microwave synthesis and exhibited excellent optical properties. Due to the strong quenching ability of I-, SC-dots were utilized for I- detection with high sensitivity and excellent selectivity, which offered a relatively low detection limit of 0.18 μM. This strategy was also successfully applied for I- detections in human serum and HeLa cells. The detection process is facile, highly sensitive and selective, providing a new insight into the potential applications of SC-dots for anion nanoprobe designs in clinical diagnosis and other biologically related areas. Graphical abstract.

中文翻译：

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使用功能化的碳纳米点作为荧光探针进行无标记碘化物检测。

基于对精胺官能化碳点（SC点）的直接荧光淬灭，无论是在复杂的生物流体还是活细胞中，均开发了用于碘离子（I-）检测的无标记荧光纳米探针。带正电荷的SC点是通过一步微波合成法制备的，具有出色的光学性能。由于I-的强大淬灭能力，SC点被用于I-检测，具有高灵敏度和出色的选择性，从而提供了0.18μM的相对较低的检测限。该策略也已成功应用于人血清和HeLa细胞中的I-检测。检测过程简便，高度灵敏且选择性强，为SC-点在阴离子纳米探针设计中的潜在应用在临床诊断和其他生物学相关领域提供了新的见解。