A simple, yet effective and rapid approach for the synthesis of titanium dioxide nanoparticles (TiO₂ NPs) using Streptomyces sp. HC1 was developed. The in vitro activity of TiO₂ NPs was demonstrated against human pathogenic bacteria and fungi. The synthesized nanoparticles were characterized using various methods and instruments, such as Zetasizer, X-ray diffraction, Fourier transmission infrared spectroscopy, atomic force microscopy, and scanning electron microscopy. TiO₂ NPs were observed to be spherical in shape with size in the range of 30 to 70 nm. Antimicrobial activities of TiO₂ NPs against Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 35218, Candida albicans, ATCC 10231, and Aspergillus niger ATCC 6275 were evaluated. It was observed that TiO₂ NPs showed higher antimicrobial activity against bacteria (12 mm) than against fungi. Moreover, TiO₂ NPs also showed potent antibiofilm activity against Pseudomonas aeruginosa ATCC 27853. Hence, the present study highlights the biological synthesis and characterization of TiO₂ NPs, and their applications in the field of biomedical sciences.

中文翻译：

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一种合成TiO 2纳米粒子的新方法：使用链霉菌属生物合成。HC1

一种简单，有效且快速的方法，使用链霉菌属（Streptomyces sp。）合成二氧化钛纳米颗粒（TiO ₂ NPs）。研发了HC1。证明了TiO ₂ NPs的体外抗人病原菌和真菌的活性。使用Zetasizer，X射线衍射，傅立叶透射红外光谱，原子力显微镜和扫描电子显微镜等各种方法和仪器对合成的纳米颗粒进行表征。观察到TiO ₂ NP为球形，尺寸在30至70nm范围内。TiO ₂ NPs对金黄色葡萄球菌ATCC 29213，大肠杆菌的抗菌活性评价了ATCC 35218，白色念珠菌，ATCC 10231和黑曲霉ATCC 6275。观察到，TiO ₂ NPs对细菌（12 mm）的抗菌活性高于对真菌的抗菌活性。此外，TiO ₂ NPs也显示出对铜绿假单胞菌ATCC 27853的有效抗生物膜活性。因此，本研究着重介绍了TiO ₂ NPs的生物合成和表征，及其在生物医学领域的应用。