In the research, two novel coordination polymers based on Cu(II) ion as the metal source and their nanostructures have been prepared via utilizing two ligands of positional isomeric pyridine carboxylic acid 2-(5-carboxypyridin-3-yl)terephthalic acid (H₃L₂) and 4-(6-carboxypyridin-3-yl)phthalic acid (H₃L₁) under the conditions of ultrasonic and solvothermal reaction, and their chemical formulas are {[Cu₃(L₁)₂(H₂O)₆](H₂O)₇}_n (1) along with {[Cu₃(L₁)₂(H₂O)₂](H₂O)₂}_n (2). In addition, the treatment effect of 1 and 2 on the human cancer in vitro and in vivo was detected. The Cell Counting Kit-8 (CCK8) method was used to determine the proliferation of human lung cancer cell line (NCI-H292) after nanoparticles 1–2 treatment, the results exhibited that nanoparticle 1 had excellent inhibitory function on NCI-H292 cell viability. Besides, ROS accumulation in the NCI-H292 cells was detected by reactive oxygen species (ROS) assay and annexin V-FITC/PI assay was conducted to detect the apoptotic cells numbers of NCI-H292 cells induced by nanoparticles 1–2 treatment. In addition, the tumor model was established in vivo and the treatment ability of the nanoparticle on NCI-H292 tumor was evaluated by measuring the mice weight and tumor volume.

中文翻译：

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Cu（II）配位聚合物纳米结构诱导人和体内肺癌细胞凋亡细胞死亡。

在这项研究中，通过利用位置异构吡啶羧酸2-（5-羧基吡啶-3-基）对苯二甲酸（H）的两个配体制备了两种以Cu（II）离子为金属源的新型配位聚合物及其纳米结构。₃ L ₂）和4-（6-羧基吡啶-3--3-基）邻苯二甲酸（H ₃ L ₁）在超声和溶剂热反应的条件下，其化学式为{[Cu ₃（L ₁）₂（H ₂ O）₆ ]（H ₂ O）₇ } _n（1）与{[Cu ₃（L ₁）₂（H ₂ O）₂ ]（H ₂ O）₂ } _n（2）。此外，还检测了1和2在体外和体内对人类癌症的治疗效果。的细胞计数试剂盒8，使用（CCK8）方法来确定人肺癌细胞系的纳米颗粒后的增殖（NCI-H292）1 - 2治疗，结果显示出该纳米粒子1对NCI-H292细胞活力具有极好的抑制作用。此外，在NCI-H292细胞ROS积累物通过活性氧检测被进行（ROS）测定和膜联蛋白V-FITC / PI法检测由纳米颗粒诱导的NCI-H292细胞的凋亡细胞数1 - 2治疗。另外，在体内建立了肿瘤模型，并且通过测量小鼠体重和肿瘤体积来评估纳米颗粒对NCI-H292肿瘤的治疗能力。