Abstract

The impact of graphene oxide (GO) on normal cells has been widely investigated. However, much less is known on its effect on cancer cells. Herein, GO nanosheets were incorporated into electrospun cellulose acetate (CA) microfibers. The GO-incorporated CA (GO/CA) microfibers were combined with bacterial cellulose (BC) nanofibers via in situ biosynthesis to obtain the nano-microfibrous scaffolds. The GO/CA–BC scaffolds were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), Raman spectroscopy, and X-ray photoelectron spectroscopy (XPS). The GO/CA–BC scaffolds were used for breast cancer cell culture to evaluate the effect of GO on cancer cell behavior. Fluorescence images revealed large multicellular clusters on the surface of GO/CA–BC scaffolds. Compared to the bare CA–BC scaffold, the GO/CA–BC scaffolds not only showed enhanced mechanical properties but also improved cell proliferation. It is expected that the GO/CA–BC scaffolds would provide a suitable microenvironment for the culture of cancer cells which is necessary for drug screening and cell biology study.

Graphic abstract

摘要

氧化石墨烯（GO）对正常细胞的影响已被广泛研究。但是，关于其对癌细胞的作用所知甚少。在此，将GO纳米片结合到静电纺丝的醋酸纤维素（CA）微纤维中。通过原位生物合成将掺有GO的CA（GO / CA）超细纤维与细菌纤维素（BC）纳米纤维结合起来，获得纳米微纤维支架。GO / CA-BC支架的特征在于扫描电子显微镜（SEM），傅立叶变换红外光谱（FTIR），热重分析（TGA），拉曼光谱和X射线光电子能谱（XPS）。GO / CA-BC支架用于乳腺癌细胞培养，以评估GO对癌细胞行为的影响。荧光图像显示GO / CA-BC支架表面上有大型多细胞簇。与裸露的CA-BC支架相比，GO / CA-BC支架不仅显示出增强的机械性能，而且还改善了细胞增殖。预计GO / CA-BC支架将为癌细胞的培养提供合适的微环境，这对于药物筛选和细胞生物学研究是必不可少的。

图形摘要