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Impact of the used solvent on the reconstitution efficiency of evaporated biosamples for untargeted metabolomics studies.
Metabolomics ( IF 3.6 ) Pub Date : 2020-03-02 , DOI: 10.1007/s11306-019-1631-1
Sascha K Manier 1 , Markus R Meyer 1
Affiliation  

INTRODUCTION Untargeted metabolomics intends to objectively analyze a wide variety of compounds. Their diverse physicochemical properties make it difficult to choose an appropriate reconstitution solvent after sample evaporation without influencing the chromatography or hamper column sorbent integrity. OBJECTIVES The study aimed to identify the most appropriate reconstitution solvent for blood plasma samples in terms of feature recovery, four endogenous compounds, and one selected internal standard. METHODS We investigated several reconstitution solvent mixtures containing acetonitrile and methanol to resolve human plasma extract and evaluated them concerning the peak areas of tryptophan-d5, glucose, creatinine, palmitic acid, and the phophatidylcholine PC(P-16:0/P-16:0), as well as the total feature count RESULTS: Results indicated that acetonitrile containing 30% methanol was best suited to match all tested criteria at least for human blood plasma samples. CONCLUSION Despite identifying the mixture of acetonitrile and methanol being suitable as solvent for human blood plasma extracts, we recommend to systematically test for an appropriate reconstitution solvent for each analyzed biomatrix.

中文翻译:

无目标代谢组学研究中所用溶剂对蒸发生物样品的重建效率的影响。

引言非靶向代谢组学旨在客观地分析各种化合物。它们的多种理化特性使样品蒸发后难以选择合适的重构溶剂而不影响色谱或妨碍色谱柱吸附剂的完整性。目的本研究旨在从特征恢复,四种内源性化合物和一种选定的内标物方面确定最适合血浆样品的重构溶剂。方法我们研究了几种含有乙腈和甲醇的重构溶剂混合物来解析人血浆提取物,并评估了它们与色氨酸-d5,葡萄糖,肌酐,棕榈酸和磷脂酰胆碱PC(P-16:0 / P-16: 0),以及总功能计数结果:结果表明,至少对于人血浆样品,含有30%甲醇的乙腈最适合满足所有测试标准。结论尽管确定了乙腈和甲醇的混合物适合作为人体血浆提取物的溶剂,但我们建议系统地测试每种分析的生物基质的合适重构溶剂。
更新日期:2020-03-03
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