Licorice is a widely used herbal medicine for the treatment of various diseases in southern Europe and parts of Asia. It has been reported that the isoliquiritin (ISL) from Glycyrrhiza root has the activity of promoting angiogenesis. The purpose of this study was to investigate the effect of ISL on the wound healing activity of zebrafish and its mechanism. 6-month-old zebrafish were injured in the skin (2 mm in diameter) and then treated with ISL. By measuring wound size and by histological examination, we found that ISL improved wound healing. In addition, 4-day-old zebrafish embryos of double transgenic line [Tg(fli-1:EGFP)]/[Tg(mpeg:mCherry)] were suffered from tissue traumas and then treated with ISL. Through fluorescent microscopy, we found that ISL promoted macrophage recruitment and angiogenesis in the wound area. Through qPCR analysis, we found that ISL up-regulated the expression of genes related to inflammation and angiogenesis in zebrafish embryos. These results showed that ISL could promote inflammatory response and angiogenesis, which played key roles in promoting wound healing. Therefore, ISL can be used as a promising candidate to promote wound healing.

欧亚甘草是一种广泛使用的草药，用于治疗南欧和亚洲部分地区的各种疾病。据报道，来自甘草根的异寡糖（ISL）具有促进血管生成的活性。这项研究的目的是调查ISL对斑马鱼伤口愈合活性的影响及其机制。6个月大的斑马鱼在皮肤（直径2毫米）中受伤，然后用ISL治疗。通过测量伤口大小和组织学检查，我们发现ISL改善了伤口愈合。此外，双转基因系[Tg（fli-1：EGFP）] / [Tg（mpeg：mCherry）]遭受组织创伤，然后接受ISL治疗。通过荧光显微镜，我们发现ISL促进了伤口区域的巨噬细胞募集和血管生成。通过qPCR分析，我们发现ISL上调了斑马鱼胚胎中与炎症和血管生成有关的基因的表达。这些结果表明，ISL可以促进炎症反应和血管生成，这在促进伤口愈合中起关键作用。因此，ISL可以用作促进伤口愈合的有希望的候选者。