The ever-growing threats of multidrug-resistant bacterial infection and chronic wound healing have created an imperative need for the development of novel antibacterial materials and therapeutic strategies, especially for diabetic patients infected with multidrug-resistant bacteria. In this work, the nanocomplexes named as PB@PDA@Ag were used for eradicating multidrug-resistant bacteria and accelerating wound healing of MRSA-infected diabetic model with the assistance of laser irradiation. In vitro results revealed that the combinational strategy exerted a synergistic effect for anti-MRSA through disrupting cell integrity, producing ROS, declining ATP, and oxidizing GSH, comparing with PB@PDA@Ag or NIR laser irradiation alone. Moreover, in vivo assay demonstrated that this system effectively accelerated MRSA-infected diabetic wound healing by mitigating local inflammatory response and up-regulating VEGF expression on the wound bed. Meanwhile, satisfactory biocompatibility and negligible damage to major organs were observed. Altogether, the aforementioned results indicate that the combinational therapy of PB@PDA@Ag and NIR irradiation shows a great potential application in the field of clinic infection.

多重耐药细菌感染和慢性伤口愈合的威胁日益增长，迫切需要开发新型抗菌材料和治疗策略，尤其是对于患有多重耐药细菌感染的糖尿病患者。在这项工作中，名为PB @ PDA @ Ag的纳米复合物被用于根除耐多药细菌并在激光照射的辅助下加速感染MRSA的糖尿病模型的伤口愈合。体外结果显示，与单独的PB @ PDA @ Ag或NIR激光照射相比，该组合策略通过破坏细胞完整性，产生ROS，降低ATP和氧化GSH发挥抗MRSA的协同作用。而且，体内分析表明，该系统通过减轻局部炎症反应和上调伤口床上的VEGF表达，有效地促进了MRSA感染的糖尿病伤口的愈合。同时，观察到令人满意的生物相容性和对主要器官的损害可忽略不计。总之，上述结果表明，PB @ PDA @ Ag和NIR辐射的联合治疗在临床感染领域显示出巨大的潜在应用前景。