2,5-Furandicarboxylic acid (FDCA) is a promising bio-based chemical in polymer industry. In this work, we constructed an oxidative cascade for one-pot synthesis of FDCA from 5-hydroxymethylfurfural (HMF) by exploiting Comamonas testosteroni SC1588 cells and laccase-2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) system. HMF was oxidized to 5-hydroxymethyl-2-furancarboxylic acid (HMFCA) by the cells at neutral pH. The HMFCA formation shifted pH of the reaction mixture to acidic range, which favored laccase-TEMPO catalytic oxidation. 5-Formyl-2-furancarboxylic acid derived from HMFCA via laccase-TEMPO catalysis was converted to FDCA by the cells. FDCA was obtained in an 87% yield within 36 h, providing a productivity of around 0.4 g/L h.

2,5-呋喃二甲酸（FDCA）是聚合物工业中一种有前途的生物基化学品。在这项工作中，我们通过利用Comamonas testosteroni SC1588细胞和漆酶2,2,6,6-四甲基哌啶-1-氧基（TEMPO）系统，构建了从5-羟甲基糠醛（HMF）一锅合成FDCA的氧化级联反应。在中性pH下，HMF被细胞氧化为5-羟甲基-2-呋喃甲酸（HMFCA）。HMFCA的形成将反应混合物的pH移至酸性范围，这有利于漆酶-TEMPO催化氧化。通过漆酶-TEMPO催化从HMFCA衍生的5-甲酰基-2-呋喃甲酸被细胞转化为FDCA。在36小时内以87％的产率获得了FDCA，提供了约0.4 g / L h的生产率。