当前位置: X-MOL 学术Arab. J. Chem. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Inner filter effect (IFE) as a simple and selective sensing platform for detection of tetracycline using milk-based nitrogen-doped carbon nanodots as fluorescence probe
Arabian Journal of Chemistry ( IF 6 ) Pub Date : 2020-04-01 , DOI: 10.1016/j.arabjc.2020.02.013
Baraa Al-Hashimi , Khalid M. Omer , Heshu S. Rahman

Abstract A simple, selective, and sensitive turn-off fluorescent assay for detecting of tetracycline in pharmaceutical dosage form based on inner filter effect (IFE) sensing platform has been described. In this IFE sensing strategy, N-doped carbon dots (CDs) were prepared by one-pot solvothermal synthesis using milk as a precursor and were directly used as a fluorophore in IFE. The prepared CDs were characterized by common spectroscopic and microscopic techniques. The CDs exhibited excitation-wavelength dependent emission with 10% as the fluorescence quantum yield. The fluorescence of CDs was decreased in correlation to the addition of absorber (tetracycline), as the excitation spectrum of the fluorophore (CDs) matches the absorption spectrum of the absorber. The present IFE-based sensing platform showed a good linear relationship from 2.0 µM to 200 µM (R2 = 0.9960) and provided a detection limit of 0.6 µM (signal-to-noise ratio of 3). Additionally, the cytotoxic effects of CDs were determined using normal healthy male Balb/C mice model treated with various doses of CDs and at the end of the study, no mortality or even no sign of toxicity was observed at oral doses of 100 and 200 mg/kg CDs in all treated animals. The proposed nanoprobe assay is a free from interferences, low-cost, biocompatible, and accurate for the detection of tetracycline in pharmaceutical formulation.

中文翻译:

内过滤效应(IFE)作为一种简单且选择性的传感平台,用于使用牛奶基氮掺杂碳纳米点作为荧光探针检测四环素

摘要 描述了一种基于内过滤效应 (IFE) 传感平台的简单、选择性和灵敏的关闭荧光测定法,用于检测药物剂型中的四环素。在这种 IFE 传感策略中,N 掺杂碳点 (CD) 是通过使用牛奶作为前体的一锅溶剂热合成制备的,并直接用作 IFE 中的荧光团。制备的 CD 用常见的光谱和显微技术表征。CD 表现出与激发波长相关的发射,荧光量子产率为 10%。由于荧光团 (CDs) 的激发光谱与吸收剂的吸收光谱相匹配,CDs 的荧光与吸收剂(四环素)的添加相关联。目前基于 IFE 的传感平台从 2 显示出良好的线性关系。0 µM 至 200 µM (R2 = 0.9960),检测限为 0.6 µM(信噪比为 3)。此外,CDs 的细胞毒性作用是使用用不同剂量 CDs 治疗的正常健康雄性 Balb/C 小鼠模型确定的,在研究结束时,在 100 和 200 毫克的口服剂量下未观察到死亡或什至没有毒性迹象/kg 所有治疗动物的 CDs。所提出的纳米探针检测方法无干扰、成本低、生物相容性好且准确,可用于检测药物制剂中的四环素。在所有接受治疗的动物中,在 100 和 200 毫克/千克 CD 的口服剂量下没有观察到死亡或什至没有毒性迹象。所提出的纳米探针检测方法无干扰、成本低、生物相容性好且准确,可用于检测药物制剂中的四环素。在所有接受治疗的动物中,在口服剂量为 100 和 200 毫克/千克 CD 时,没有观察到死亡或什至没有毒性迹象。所提出的纳米探针检测方法无干扰、成本低、生物相容性好且准确,可用于检测药物制剂中的四环素。
更新日期:2020-04-01
down
wechat
bug