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Yeast display platform technology to prepare oral vaccine against lethal H7N9 virus challenge in mice.
Microbial Cell Factories ( IF 6.4 ) Pub Date : 2020-03-02 , DOI: 10.1186/s12934-020-01316-1
Han Lei 1 , Bowen Xie 1 , Tong Gao 1 , Qianhong Cen 1 , Yi Ren 1
Affiliation  

BACKGROUND Existing methods for preparing influenza vaccines pose the greatest challenge against highly pandemic avian influenza H7N9 outbreak in the poultry and humans. Exploring a new strategy for manufacturing and delivering a safe and effective H7N9 vaccine is needed urgently. RESULTS An alternative approach is to develop an influenza H7N9 oral vaccine based on yeast display technology in a timely manner. Hemagglutinin (HA) of A/Anhui/1/2013 (AH-H7N9) is used as a model antigen and characterized its expression on the surface of Saccharomyces cerevisiae (S.cerevisiae) EBY 100. Mice administrated orally with S.cerevisiae EBY100/pYD5-HA produced significant titers of IgG antibody as well as significant amounts of cytokines IFN-γ and IL-4. Importantly, S.cerevisiae EBY100/pYD5-HA could provide effective immune protection against homologous A/Anhui/1/2013 (AH-H7N9) virus challenge. CONCLUSIONS Our findings suggest that platform based on yeast surface technology provides an alternative approach to prepare a promising influenza H7N9 oral vaccine candidate that can significantly shorten the preparedness period and result in effective protection against influenza A pandemic.

中文翻译:

酵母展示平台技术,用于制备针对小鼠中的致命H7N9病毒攻击的口服疫苗。

背景技术现有的制备流感疫苗的方法对在家禽和人类中高流行性禽流感H7N9暴发提出了最大的挑战。迫切需要探索一种新的策略来生产和提供安全有效的H7N9疫苗。结果另一种方法是基于酵母展示技术及时开发H7N9流感口服疫苗。使用A / Anhui / 1/2013(AH-H7N9)的血凝素(HA)作为模型抗原,并表征其在酿酒酵母(S.cerevisiae)EBY 100表面上的表达。与S.cerevisiae EBY100 /一起口服的小鼠pYD5-HA产生了显着效价的IgG抗体以及大量的细胞因子IFN-γ和IL-4。重要的是,S。啤酒酵母EBY100 / pYD5-HA可提供针对同源A / Anhui / 1/2013(AH-H7N9)病毒攻击的有效免疫保护。结论我们的研究结果表明,基于酵母表面技术的平台为制备有希望的H7N9流感口服疫苗候选药物提供了另一种方法,该候选疫苗可显着缩短准备时间并有效抵御A型流感大流行。
更新日期:2020-04-22
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