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Fine mapping of leaf rust resistance gene Rph13 from wild barley.
Theoretical and Applied Genetics ( IF 5.4 ) Pub Date : 2020-03-02 , DOI: 10.1007/s00122-020-03564-6
Matthias Jost 1 , Davinder Singh 2 , Evans Lagudah 1 , Robert F Park 2 , Peter Dracatos 2
Affiliation  

Fine mapping of the barley leaf rust resistance locus Rph13 on chromosome 3HL facilitates its use in breeding programs through marker-assisted selection. Barley leaf rust (BLR-caused by Puccinia hordei) is a widespread fungal disease that can be effectively controlled by genetic resistance. There is an ongoing need to both diversify and genetically characterise resistance loci to provide effective and durable control given the ongoing threat of rapidly evolving P. hordei populations. Here, we report on the molecular genetic characterisation of the Rph13 locus, originally derived from wild barley and transferred to barley accession Berac (then referred to as PI 531849). The 2017 reference genome of cv. Morex was used as a road map to rapidly narrow both a genetic and physical intervals around the Rph13 resistance locus. Using recombination-based mapping, we narrowed the physical interval to 116.6 kb on chromosome 3H in a segregating population of a cross of the Rph13 carrying resistant line PI 531849 with the leaf rust-susceptible cultivar Gus. We identified two nucleotide-binding leucine-rich repeat genes as likely candidates for the Rph13 resistance. Sequences from the candidate genes enabled the development of a KASP marker that distinguished resistant and susceptible progeny and was found to be predictive and useful for MAS.

中文翻译:

野生大麦抗叶锈病基因Rph13精细定位[J].

在染色体 3HL 上的大麦叶锈病抗性基因座 Rph13 的精细定位有助于通过标记辅助选择将其用于育种计划。大麦叶锈病(由 Puccinia hordei 引起的 BLR)是一种广泛传播的真菌病害,可以通过遗传抗性得到有效控制。鉴于迅速进化的大麦大麦芽孢杆菌种群的持续威胁,目前需要对抗性基因座进行多样化和基因表征,以提供有效和持久的控制。在这里,我们报告了 Rph13 基因座的分子遗传特征,该基因座最初来自野生大麦并转移到大麦品种 Berac(当时称为 PI 531849)。cv. 2017 年参考基因组。Morex 被用作快速缩小 Rph13 抗性基因座周围的遗传和物理间隔的路线图。使用基于重组的作图,我们将携带 Rph13 的抗性品系 PI 531849 与叶锈病易感品种 Gus 杂交的分离种群中,将 3H 染色体上的物理间隔缩小到 116.6 kb。我们确定了两个核苷酸结合的富含亮氨酸的重复基因作为 Rph13 抗性的可能候选者。来自候选基因的序列能够开发出区分抗性和易感后代的 KASP 标记,并被发现对 MAS 具有预测性和有用性。
更新日期:2020-03-02
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