In this work, dual drug loaded in chitosan/dextran sulfate/chitosan (CS/DEX/CS) nanoparticles was synthesized by layer-by-layer (LBL) self-assembly technique for use in anti-cancer drug delivery. The nanoparticles were characterized in terms of particle size, zeta-potential, encapsulation efficiency and morphology (SEM and TEM). The in vitro release of the dual drugs, inner PTX and outer 5-Fu, from the CS-PTX/EX/CS-5Fu nanoparticles with different numbers of CS and DEX layers and different PBS was characterized. The results revealed that the pH-sensitive dual drug loaded nanoparticles exhibited a controlled release profile, and the release mechanism followed Two-phase kinetic model for PTX and Higuchi model for 5-Fu. Subsequently the cytotoxicity of nanoparticles was evaluated against HepG2 cells using MTT and apoptosis assay, resulting in synergistic effects between dual drugs and enhanced inhibition to cancer cells. Cellular uptake studies demonstrated efficient internalization of PTX and 5-Fu in HepG2 cells. Therefore the dual drug loaded CS/DEX/CS nanoparticles had good prospects for the biomedical delivery application.

中文翻译：

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壳聚糖/硫酸葡聚糖/壳聚糖的聚电解质三层纳米颗粒，用于双重药物递送。

在这项工作中，通过层（LBL）自组装技术合成了壳聚糖/硫酸葡聚糖/壳聚糖（CS / DEX / CS）纳米粒子中负载的双重药物，以用于抗癌药物递送。根据粒径，ζ电位，包封效率和形态（SEM和TEM）表征纳米颗粒。表征了具有不同CS和DEX层数以及不同PBS的CS-PTX / EX / CS-5Fu纳米颗粒对内药物PTX和外部5-Fu的体外释放。结果表明，pH敏感的双重药物负载的纳米颗粒表现出受控的释放曲线，并且释放机理遵循PTX的两相动力学模型和5-Fu的Higuchi模型。随后，使用MTT和凋亡测定法评估了纳米颗粒对HepG2细胞的细胞毒性，导致双重药物之间具有协同作用，并增强了对癌细胞的抑制作用。细胞摄取研究证明HepG2细胞中PTX和5-Fu的有效内在化。因此，载有双重药物的CS / DEX / CS纳米颗粒具有良好的生物医学递送应用前景。