ABSTRACT In view of the increasing interest in the occurrence and spread of antibiotic-resistant bacteria due to wastewater treatment systems into the environment, total colony counts and antibiotic-resistant bacteria were determined in regard to a conventional wastewater treatment plant and its upgrade with a wastewater ozonation. To cope with the elimination of conventionally not sufficiently decimated micropollutants, the Eifel-Rur Waterboard built a full-scale ozonation plant at the stream Wurm, which is strongly influenced by WWTP discharge. To evaluate the effect of wastewater ozonation on the receiving water’s biocenosis, extensive monitoring of the WWTP and its receiving water is performed before and after implementation of ozonation treatment and in preliminary pilot-scale ozonation experiments. Total colony counts showed no significant difference between the stream Wurm upstream and downstream of the WWTP and were slightly below the average of comparable investigations. Antibiotic resistances showed only a little differences between WWTP and the stream samples. Furthermore, no accumulation of antibiotic resistances was found at the conventional WWTP. Pilot-scale ozonation yielded a reduction of total colony counts of fecal indicator bacteria Escherichia coli and Enterococci after ozone treatment. The pilot-scale experiments gave no indication that ozone treatment leads to a rise in antibiotic resistances against selected antibiotics of different antibiotic classes.

中文翻译：

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常规废水处理和废水臭氧化过程中的微生物计数和抗生素耐药性

摘要 鉴于由于废水处理系统对环境中的抗生素耐药菌的发生和传播越来越受到关注，因此确定了传统废水处理厂及其使用废水升级的总菌落计数和抗生素耐药菌。臭氧化。为了应对传统上未充分去除的微污染物的消除，Eifel-Rur Waterboard 在受 WWTP 排放强烈影响的 Wurm 溪流建造了一个全面的臭氧化工厂。为了评估废水臭氧化对接收水生物群落的影响，在实施臭氧处理之前和之后以及初步中试规模的臭氧化实验中，对污水处理厂及其接收水进行了广泛的监测。总菌落数显示在污水处理厂上游和下游的河流亚龙之间没有显着差异，略低于可比调查的平均值。污水处理厂和溪流样品之间的抗生素抗性仅显示出很小的差异。此外，在常规污水处理厂没有发现抗生素抗性的积累。臭氧处理后，中试规模的臭氧处理减少了粪便指示菌大肠杆菌和肠球菌的总菌落计数。中试规模的实验没有表明臭氧处理会导致对不同抗生素类别的选定抗生素的抗生素耐药性增加。污水处理厂和溪流样品之间的抗生素抗性仅显示出很小的差异。此外，在常规污水处理厂没有发现抗生素抗性的积累。臭氧处理后，中试规模的臭氧处理减少了粪便指示菌大肠杆菌和肠球菌的总菌落计数。中试规模的实验没有表明臭氧处理会导致对不同抗生素类别的选定抗生素的抗生素耐药性增加。污水处理厂和溪流样品之间的抗生素抗性仅显示出很小的差异。此外，在常规污水处理厂没有发现抗生素抗性的积累。臭氧处理后，中试规模的臭氧处理减少了粪便指示菌大肠杆菌和肠球菌的总菌落计数。中试规模的实验没有表明臭氧处理会导致对不同抗生素类别的选定抗生素的抗生素耐药性增加。