The cellulolytic enzymes from filamentous fungi are widely used for production of biofuels. Molecular biological engineering of fungal strains has been applied to improve the cellulase production in the recent years. The VIB gene affects production of cellulase and known as a new transcription factor. Based on a fast-growing strain Trichoderma orientalis EU7-22, we constructed two recombinant strains that knockout the VIB gene and overexpressed the VIB gene and the effects of the VIB gene on cellulase production under induction conditions were also investigated. Under the condition of induction by avicel and wheat bran, the cellulase activity of the recombinant −VIB strain was almost undetectable, while that of the +VIB strain was greatly improved. FPAase, CMCase, pNPCase, and pNPGase in the crude enzyme produced by the +VIB strain are 1.51, 41.10, 0.86, and 3.47 IU ml⁻¹, respectively, which increased 92, 34, 87, and 38% compared to the parent strain. Therefore, we believe that the VIB gene has an important effect on the cellulase of T. orientalis and overexpression of the VIB gene will increase the cellulase production. This study provided guidance for improving the cellulase production of T. orientalis.

来自丝状真菌的纤维素分解酶被广泛用于生产生物燃料。近年来，真菌菌株的分子生物学工程已被用于改善纤维素酶的生产。VIB基因影响纤维素酶的产生，被称为新的转录因子。基于快速生长的东方木霉菌株在EU7-22中，我们构建了两个敲除VIB基因并过表达VIB基因的重组菌株，并研究了VIB基因对诱导条件下纤维素酶生产的影响。在由avicel和麦麸诱导的条件下，重组-VIB菌株的纤维素酶活性几乎检测不到，而+ VIB菌株的纤维素酶活性大大提高。+ VIB菌株产生的粗酶中的FPAase，CMCase，pNPCase和pNPGase分别为1.51、41.10、0.86和3.47 IU ml ^-1，与亲本菌株相比增加了92％，34、87和38％ 。因此，我们认为VIB基因对东方锥虫的纤维素酶具有重要作用，而VIB基因的过表达将增加纤维素酶的产量。这项研究为改善东方。纤维素酶生产提供了指导。