Due to the depletion of fossil fuel resources and concern about increasing atmospheric CO2 levels, the production of microbial oil as source for energy and chemicals is considered as a sustainable alternative. A promising candidate strain for the production of microbial oil is the oleaginous yeast Schwanniomyces occidentalis CBS 2864. To compete with fossil resources, cultivation and processing of S. occidentalis requires improvement. Currently, different cell wall disruption techniques based on mechanical, chemical, physiological, and biological methods are being investigated using a variety of oil producing yeasts and microalgae. Most of these techniques are not suitable for upscaling because they are technically or energetically unfavorable. Therefore, new techniques have to be developed to overcome this challenge. Here, we demonstrate an effective mild enzymatic approach for cell disruption to facilitate lipid extraction from the oleaginous yeast S. occidentalis. Most oil was released by applying 187 mg L-1 tailor-made enzymes from Trichoderma harzianum CBS 146429 against the yeast cell wall of S. occidentalis at pH 5.0 and 40 °C with 4 h of incubation time after applying 1 M NaOH as a pretreatment step.

中文翻译：

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量身定制的酶的生产，可促进从油性酵母西旺氏酵母中提取脂质。

由于化石燃料资源的枯竭以及人们对大气中二氧化碳含量不断增加的关注，微生物油作为能源和化学品的来源被认为是一种可持续的替代方法。产油微生物酵母油菜Schwanniomyces occidentalis CBS 2864是生产微生物油的有前途的候选菌株。为了与化石资源竞争，西方油菜的培养和加工需要改进。当前，正在使用各种产油酵母和微藻研究基于机械，化学，生理和生物学方法的不同细胞壁破坏技术。这些技术中的大多数都不适合升级，因为它们在技术上或从能量上都是不利的。因此，必须开发新技术来克服这一挑战。这里，我们展示了一种有效的温和酶解方法，用于细胞破坏，以促进从产油酵母西方球菌中提取脂质。在使用1 M NaOH进行预处理后，在pH 5.0和40°C的条件下，通过将哈氏木霉CBS 146429的187 mg L-1特制酶施加到西方链球菌的酵母细胞壁上，并经过4 h的孵育时间，释放了大多数油脂步。