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Chemical characterization, antioxidant, enzyme inhibitory and cytotoxic properties of two geophytes: Crocus pallasii and Cyclamen cilicium.
Food Research International ( IF 8.1 ) Pub Date : 2020-02-28 , DOI: 10.1016/j.foodres.2020.109129
Gokhan Zengin 1 , Mohamad Fawzi Mahomoodally 2 , Kouadio Ibrahime Sinan 1 , Marie Carene Nancy Picot-Allain 3 , Evren Yildiztugay 4 , Zoltán Cziáky 5 , József Jekő 5 , Hammad Saleem 6 , Nafees Ahemad 7
Affiliation  

The Crocus and Cyclamen genus have been reported to possess diverse biological properties. In the present investigation, two geophytes from these genus, namely Crocus pallasi and Cyclamen cilicium have been studied. The in vitro antioxidant, enzyme inhibitory, and cytotoxic effects of the methanol extracts of Crocus pallasii and Cyclamen cilicium aerial and underground parts were investigated. Antioxidant abilities of the extracts were investigated via different antioxidant assays (metal chelating, radical quenching (ABTS and DPPH), reducing power (CUPRAC and FRAP) and phosphomolybdenum). Cholinesterases, amylase, tyrosinase, and glucosidase were used as target enzymes for detecting enzyme inhibitory abilities of the samples. Regarding the cytotoxic abilities, breast cancer cell lines (MDA-MB 231 and MCF-7) and prostate cancer cell lines (DU-145) were used. The flowers extracts of Crocus pallasii and C. cilicium possessed the highest flavonoid content. The highest phenolic content was recorded from C. cilicium root extract (47.62 mg gallic acid equivalent/g extract). Cyclamen cilicium root extract showed significantly (p<0.05) high radical scavenging (94.28 and 139.60 mg trolox equivalent [TE]/g extract, against DPPH and ABTS radicals, respectively) and reducing potential (173.30 and 109.53 mg TE/g extract, against CUPRAC and FRAP, respectively). The best acetylcholinesterase, glucosidase and tyrosinase inhibition was observed in C. cilicium root (4.46 mg GALAE/g; 15.75 mmol ACAE/g; 136.99 mg KAE/g, respectively). Methanolic extracts of C. pallasii and C. cilicium showed toxicity against breast cancer cell lines. In light of the above findings, C. cilicium might be considered as an interesting candidate in the development of anti-cancer agent coupled with antioxidant properties.



中文翻译:

两种土生植物的化学特性,抗氧化剂,酶抑制和细胞毒性特性:番红花和仙客来。

番红花仙客来属已报道具有不同的生物学特性。在本研究中,已经研究了来自这两个属的两种植物,即番红花仙客来。的体外抗氧化剂,甲醇提取物的酶抑制,并且细胞毒性作用番pallasii仙客来cilicium地上和地下部分进行了研究。提取物的抗氧化能力通过不同的抗氧化剂测定(金属螯合,自由基淬灭(ABTS和DPPH),还原能力(CUPRAC和FRAP)和磷钼)。胆碱酯酶,淀粉酶,酪氨酸酶和葡糖苷酶被用作目标酶,以检测样品的酶抑制能力。关于细胞毒性能力,使用了乳腺癌细胞系(MDA-MB 231和MCF-7)和前列腺癌细胞系(DU-145)。红花纤毛锦葵的花提取物中黄酮含量最高。从纤毛棒根提取物中记录到最高的酚含量(47.62 mg没食子酸当量/ g提取物)。仙客来纤毛根提取物显着(p<0.05)高自由基清除率(94.28和139.60 mg trolox当量[TE] / g提取物,分别针对DPPH和ABTS自由基)和还原电位(173.30和109.53 mg TE / g提取物,分别针对CUPRAC和FRAP)。在纤毛棒菌根中观察到最佳的乙酰胆碱酯酶,葡糖苷酶和酪氨酸酶抑制作用(分别为4.46 mg GALAE / g; 15.75 mmol ACAE / g; 136.99 mg KAE / g)。C. pallasiiC. cilicium的甲醇提取物表现出对乳腺癌细胞系的毒性。鉴于以上发现,纤毛衣藻可被认为是抗癌剂与抗氧化剂性质结合发展的有趣候选者。

更新日期:2020-03-02
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