Key objective of this work was to evaluate the use of cellulosic fraction from sugarcane straw pretreated by liquid hot water (LHW) for butanol production via acetone-butanol-ethanol (ABE) fermentation. Separated hydrolysis and fermentation (SHF), and pre-saccharification and simultaneous saccharification and fermentation (PSSF) were investigated at 10 and 15 % w/v biomass loading. For 15 % w/v, the synergistic effect of weak acids and phenolic compounds made the sugarcane straw hydrolysate poorly fermentable. The 10 % w/v solid load was more favorable (∼ 4-fold higher) in both SHF and PSSF strategies with respect to the ABE production, without including a detoxification step. However, PSSF achieved higher ABE titer (10.5 g/L – SHF; 13.5 g/L – PSSF) and productivity (0.09 g/(L.h) – SHF; 0.14 g/(L.h) – PSSF) when compared with SHF. Using best condition (PSSF at 10 % w/v), it would be possible to estimate a yield of 169 L ABE per ton pretreated sugarcane straw (or 84.5 L ABE per ton of raw sugarcane straw), containing 65 L acetone, 95 L butanol, and 9 L ethanol. This result represents a process efficiency of 28 %, based on carbohydrates content in raw material.

这项工作的主要目的是评估通过液体热水（LHW）预处理的甘蔗秸秆中的纤维素级分通过丙酮-丁醇-乙醇（ABE）发酵生产丁醇的用途。在10％和15％w / v的生物量负载下，研究了分离的水解和发酵（SHF），预糖化以及同时糖化和发酵（PSSF）。对于15％w / v，弱酸和酚类化合物的协同作用使甘蔗秸秆水解产物难以发酵。在不包括解毒步骤的情况下，相对于ABE生产，在SHF和PSSF策略中10％w / v的固含量更有利（约高4倍）。但是，与SHF相比，PSSF的ABE效价更高（10.5 g / L – SHF； 13.5 g / L – PSSF）和生产率（0.09 g /（Lh）– SHF； 0.14 g /（Lh）– PSSF）。在最佳条件下（PSSF为10％w / v），估计每吨预处理甘蔗秸秆含65 L丙酮，95 L甘蔗秸秆的产量为169 L ABE（每吨原蔗糖秸秆为84.5 L ABE）丁醇和9升乙醇。基于原料中的碳水化合物含量，该结果表示工艺效率为28％。