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Sensitive and selective detection of puerarin based on the hybrid of reduced graphene oxide and molecularly imprinted polymer.
Journal of Pharmaceutical and Biomedical Analysis ( IF 3.4 ) Pub Date : 2020-02-29 , DOI: 10.1016/j.jpba.2020.113221
Haifeng Li 1 , Shuang Wang 1 , Fan Cui 1 , Beibei Zhuo 1 , Shuang Wang 1 , Chenrui Zhao 1 , Weilu Liu 1
Affiliation  

Puerarin, an important isoflavone, has been widely used for the treatment of angina and hypertension. In this work, we developed a novel electrochemical sensor for the detection of puerarin based on the hybrid of reduced graphene oxide (RGO) and molecularly imprinted polymer (MIP). The RGO/MIP sensor functions by target puerarin recognition and electro-oxidization via a two-proton and two-electron process, enabling the detection of puerarin with good selectivity and high sensitivity. The MIP layer was integrated on the surface of RGO by the electro-co-polymerization of o-phenylenediamine (monomer) and puerarin (template), resulting in high surface area, binding capacity, good conductivity and faster mass transfer. The nanostructure of the RGO/MIP hybrid was demonstrated using scanning electron microscopy (SEM) and atomic force microscopy (AFM). Experimental conditions involved in the sensor fabrication process were evaluated. Under the optimized condition, a wide linear range (0.02 μM ∼ 40 μM) and a low detection limit (0.006 μM) were achieved. The sensor was applied to detect puerarin in human urine and injection samples, and the result was comparable with that of the gold standard method of high-performance liquid chromatography (HPLC), indicating a promise in the further application to pharmacokinetics or therapeutic drug monitoring.

中文翻译:

基于还原型氧化石墨烯和分子印迹聚合物的混合,对葛根素进行灵敏和选择性的检测。

葛根素是一种重要的异黄酮,已被广泛用于治疗心绞痛和高血压。在这项工作中,我们基于还原型氧化石墨烯(RGO)和分子印迹聚合物(MIP)的混合物,开发了一种用于检测葛根素的新型电化学传感器。RGO / MIP传感器的功能是通过两个质子和两个电子过程识别目标葛根素并进行电氧化,从而能够以良好的选择性和高灵敏度检测葛根素。通过邻苯二胺(单体)和葛根素(模板)的电共聚,将MIP层整合到RGO的表面,从而获得高表面积,结合能力,良好的电导率和更快的质量转移。使用扫描电子显微镜(SEM)和原子力显微镜(AFM)证明了RGO / MIP杂化物的纳米结构。评估了传感器制造过程中涉及的实验条件。在优化条件下,可实现宽线性范围(0.02μM〜40μM)和低检测限(0.006μM)。该传感器用于检测人类尿液和注射样品中的葛根素,其结果与高效液相色谱(HPLC)的金标准方法相当,这表明在进一步应用于药代动力学或治疗药物监测中具有希望。
更新日期:2020-03-02
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