Comparative Biochemistry and Physiology D: Genomics & Proteomics ( IF 3 ) Pub Date : 2019-05-29 , DOI: 10.1016/j.cbd.2019.100596 Andrea Rivas-Aravena , Marcia Fuentes-Valenzuela , Sebastian Escobar-Aguirre , Cristian Gallardo-Escarate , Alfredo Molina , Juan Antonio Valdés
Flavobacterium psychrophilum is the etiologic agent of rainbow trout fry syndrome (RTFS). This pathogen infects a wide variety of salmonid species during freshwater stages, causing significant losses in the aquaculture industry. Rainbow trout (Oncorhynchus mykiss) infected with F. psychrophilum, presents as the main external clinical sign ulcerative lesions and necrotic myositis in skeletal muscle. We previously reported the in vitro cytotoxic activity of F. psychrophilum on rainbow trout myoblast, however little is known about the molecular mechanisms underlying the in vivo pathogenesis in skeletal muscle.
In this study, we examined the transcriptomic profiles of skeletal muscle tissue of rainbow trout intraperitoneally challenged with low infection dose of F. psychrophilum. Using high-throughput RNA-seq, we found that 233 transcripts were up-regulated, mostly associated to ubiquitin mediated proteolysis and apoptosis. Conversely, 189 transcripts were down-regulated, associated to skeletal muscle contraction. This molecular signature was consistent with creatine kinase activity in plasma and oxidative damage in skeletal muscle. Moreover, the increased caspase activity suggests as a whole skeletal muscle atrophy induced by F. psychrophilum. This study offers an integrative analysis of the skeletal muscle response to F. psychrophilum infection and reveals unknown aspects of its pathogenesis in rainbow trout.
中文翻译:
虹鳟鱼的转录响应(虹鳟)骨骼肌黄杆菌psychrophilum
嗜冷黄杆菌是虹鳟鱼苗综合征(RTFS)的病原体。这种病原体在淡水阶段会感染多种鲑鱼物种,在水产养殖业中造成重大损失。虹鳟鱼(虹鳟)感染F. psychrophilum,呈现为主要外部临床体征溃疡性病变和坏死性肌炎骨骼肌。我们以前曾报道过嗜铬镰刀菌对虹鳟成肌细胞的体外细胞毒性活性,但是对骨骼肌体内致病机理的分子机制了解甚少。
在这项研究中,我们检查了腹膜内挑战低感染剂量的嗜热镰刀菌虹鳟的骨骼肌组织的转录组谱。使用高通量RNA-seq,我们发现233个转录本被上调,主要与泛素介导的蛋白水解和细胞凋亡有关。相反,下调了189个转录本,与骨骼肌收缩有关。这种分子特征与血浆中的肌酸激酶活性和骨骼肌的氧化损伤相一致。此外,增加的半胱天冬酶活性暗示由嗜冷镰刀菌诱导的整个骨骼肌萎缩。这项研究对骨骼肌对嗜铬镰刀菌的反应进行了综合分析。 感染并揭示虹鳟鱼发病机理的未知方面。