ABSTRACT A novel rhodamine-based chemosensor (R) was designed and synthesised for selective recognition of Hg2+ ion in real water samples collected from different places. The chemosensor was prepared in green condition with high yield. The selectivity of R was examined with various metal ions, among which only Hg2+ was identified selectively with off–on mechanism along with enhancement of fluorescence. Metal ions recognition has been carried out using UV–vis and fluorescence studies taking µM concentration of chemosensor R in HEPES buffer. The detection limit of R was calculated and found to be 4.4 × 10–9 M. Quantum chemical (DFT) calculation was carried out in order to acquire knowledge about the stability of R in presence of Hg2+ ions. Cell viability and fluorescence microscopic experiments showed R as cytocompatible and can be used as a fluorescent probe for detecting Hg2+ in living cells. Graphical Abstract Detection of Hg2+ ion using highly selective fluorescent chemosensor in real water sample and in-vitro cell study upon breast adenocarcinoma (MCF-7) Ashish Kumara, Sumit Kumar Hirab, Partha Pratim Mannac and Swapan Deya*

中文翻译：

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使用高选择性荧光化学传感器检测实际水样中的 Hg2+ 离子和体外细胞研究对乳腺癌 (MCF-7)

摘要设计并合成了一种新型的基于罗丹明的化学传感器 (R)，用于选择性识别从不同地方采集的真实水样中的 Hg2+ 离子。化学传感器在绿色条件下制备，产量高。用各种金属离子检查了 R 的选择性，其中只有 Hg2+ 被选择性地鉴定为具有关断机制以及荧光增强。金属离子识别已使用 UV-vis 和荧光研究进行，在 HEPES 缓冲液中采用 μM 浓度的化学传感器 R。计算了 R 的检测限，发现为 4.4 × 10–9 M。进行了量子化学 (DFT) 计算，以了解 R 在 Hg2+ 离子存在下的稳定性。细胞活力和荧光显微镜实验表明 R 具有细胞相容性，可用作检测活细胞中 Hg2+ 的荧光探针。图形摘要 使用高选择性荧光化学传感器检测实际水样中的 Hg2+ 离子以及体外细胞研究对乳腺癌 (MCF-7) Ashish Kumara、Sumit Kumar Hirab、Partha Pratim Mannac 和 Swapan Deya*