SNP-Based Kompetitive Allele Specific PCR (KASPTM) Method for the Qualification and Quantification of Hop Varieties,Journal of the American Society of Brewing Chemists

当前位置： X-MOL 学术 › J. Am. Soc. Brew. Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

SNP-Based Kompetitive Allele Specific PCR (KASPTM) Method for the Qualification and Quantification of Hop Varieties
Journal of the American Society of Brewing Chemists ( IF 2 ) Pub Date : 2018-07-03 , DOI: 10.1080/03610470.2018.1483702
Peiji Jiang _{1,

2} , Tao Song ₂ , Wei Jiang ₂ , Deliang Wang ₂ , Biao Pu ₁ , Chunguang Luan ₂

Affiliation

Abstract There are several studies focused on single nucleotide polymorphisms (SNPs) of hops. However, to date, no feasible method for the qualification and quantification of hop varieties had been developed. A novel Kompetitive Allele Specific PCR (KASP) methodology for the qualification and quantification of hop varieties has been developed. One hundred and fifty gauge of high-throughput sequencing data were obtained from the ten hop varieties most commonly used in the Chinese brewing industry. After bioinformatic analysis, 2046 SNPs from sixteen hop varieties were selected from two million original SNPs for further confirmation by Sanger sequencing. Twelve SNP markers were successfully screened for the characterization and quantification of the mixed sample. The SNP markers and detection method developed provide potential means for the qualification and quantification of hop varieties in laboratories utilizing SNP marker technology.

更新日期：2018-07-03

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南

全部期刊列表>>