Abstract Precise determination of yeast physiology is crucial in both laboratories and breweries. Our aim was to identify a sensitive indicator that could demonstrate subtle physiological differences in brewer’s yeast cultures. During 4-day ageing at 25 °C, the cell viability, intracellular adenosine triphosphate (ATP) content, and intracellular pH values changed significantly. The ATP content and intracellular pH value both correlated with viability. At 4 °C, a correlation was also observed between ATP content and viability, while no correlation was found between intracellular pH value and viability. When the relationship between intracellular ATP and viability in other strains was investigated, it was found that the ATP content could reflect both viability and vitality. When viability was significantly modified, the ATP content changed considerably more than the viability. When viability remained unchanged, the significant decrease in ATP content reflected a decrease in vitality. Taken together, it was concluded that intracellular ATP content is more sensitive than the intracellular pH value and viability; thus, this parameter can help identify subtle changes in yeast environmental responses when other commonly used indicators remain unchanged.

中文翻译：

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细胞内三磷酸腺苷 (ATP) 含量敏感地反映了酵母生理学的细微差异

摘要 酵母生理学的精确测定对于实验室和啤酒厂都至关重要。我们的目标是确定一种敏感指标，可以证明啤酒酵母培养物中的细微生理差异。在 25 °C 下老化 4 天期间，细胞活力、细胞内三磷酸腺苷 (ATP) 含量和细胞内 pH 值发生显着变化。ATP 含量和细胞内 pH 值均与活力相关。在 4 °C 时，还观察到 ATP 含量与活力之间存在相关性，而细胞内 pH 值与活力之间未发现相关性。当研究其他菌株细胞内ATP和活力之间的关系时，发现ATP含量可以反映活力和活力。当生存能力显着改变时，ATP 含量的变化远大于生存力。当活力保持不变时，ATP含量的显着下降反映了活力的下降。综合来看，细胞内ATP含量比细胞内pH值和活力更敏感；因此，当其他常用指标保持不变时，该参数可以帮助识别酵母环境响应的细微变化。