Journal of Macromolecular Science Part B-Physics ( IF 1.4 ) Pub Date : 2019-06-08 , DOI: 10.1080/00222348.2019.1615688 Yang Liu 1, 2, 3 , Ying Lv 1, 2, 3 , Meiwen An 2, 3 , Fen Li 1, 2, 3 , Ying Lu 4 , Jianbo Song 4
Vacuum freeze-drying was used to prepare chitosan-gelatin (CG) scaffolds from hydrogels, with glutaraldehyde (GA) used as a crosslinker. The effects of the changes in volume ratios of the 2 wt% CG and GA solutions on scaffold performance were studied. The ratio of chitosan to gelatin solution volumes, vr(C/G), was adjusted to 1/2 or 1/1, with the 0.25 wt% GA volume at 3, 6, or 8% of the CG/GA volume. Six groups of CG scaffolds were fabricated and the scaffolds performance compared. After the cells were incubated for 4 days, hematoxylin eosin (HE) staining was used to observe the spreading of human skin keratinocyte (HaCaT) cells on these scaffolds, with the MTT method also used to detect the cells proliferation. The inhibition zone method was used on cells cultures to determine the antibacterial properties of the scaffolds against S. aureus and E. coli. Scaffolds were also examined for degradation in lysozyme and their compression properties were tested after degradation. The results showed that the HaCaT cells grew well on these scaffolds and proliferated significantly, indicating that these scaffolds possessed good cytocompatibility. With increased chitosan volume, the antibacterial properties of the scaffolds against S. aureus increased, however, there was no significant change in the antibacterial properties toward E. coli. Increased volumes of chitosan and GA decreased the scaffolds degradation rates and improved the elastic compressive moduli of the scaffolds after degradation. The scaffolds in the vr(C/G) = 1/1, 8% GA group have potential application prospects in the field of skin regeneration.
中文翻译:
壳聚糖-明胶混合支架的表征
真空冷冻干燥用于由水凝胶制备壳聚糖-明胶(CG)支架,戊二醛(GA)用作交联剂。研究了2 wt%CG和GA溶液的体积比变化对脚手架性能的影响。将壳聚糖与明胶溶液体积的比率vr(C / G)调整为1/2或1/1,其中0.25 wt%的GA体积为CG / GA体积的3、6或8%。制作了六组CG支架,并比较了支架性能。将细胞孵育4天后,使用苏木精曙红(HE)染色观察人皮肤角质形成细胞(HaCaT)在这些支架上的扩散,同时还使用MTT方法检测细胞增殖。抑制区方法用于细胞培养物中,以确定支架的抗细菌特性金黄色葡萄球菌和大肠杆菌。还检查支架在溶菌酶中的降解,并在降解后测试其压缩特性。结果表明,HaCaT细胞在这些支架上生长良好,并显着增殖,表明这些支架具有良好的细胞相容性。随着壳聚糖体积的增加,支架对金黄色葡萄球菌的抗菌性能增加,但是,对大肠杆菌的抗菌性能没有明显变化。。壳聚糖和GA体积的增加降低了支架的降解速率,并改善了降解后支架的弹性压缩模量。vr(C / G)= 1 / 1,8%GA组的支架在皮肤再生领域具有潜在的应用前景。