Biogenic amine biosensors, based on screen-printed carbon electrodes (SPCE) modified with Prussian blue (PB) and indium tin oxide nanoparticles (ITONP), are reported. PB/ITONP-modified SPCE was further modified with diamine oxidase (DAO) or monoamine oxidase (MAO) enzymes to construct the biosensors. The morphology of the modified electrodes was studied by scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX) and atomic force microscopy (AFM). Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to enlighten the electrochemical properties of the modified electrodes at each step of biosensor fabrication. Electrode surface composition and experimental conditions were optimized and analytical performance characteristics of the biosensors were studied. Several biogenic amines were tested and both biosensors responded to histamine, putrescine and cadaverine. DAO/ITONP/PB/SPCE biosensor exhibited the highest response to histamine 6.0 × 10-6-6.9 × 10-4 M with a sensitivity of 1.84 μA mM-1. On the other hand, the highest sensitivity was obtained for cadaverine with the MAO/ITONP/PB/SPCE biosensor. The analytical utility of the presented biosensors were illustrated by the determination of cadaverine and histamine in cheese sample.

中文翻译：

---

基于普鲁士蓝，铟锡氧化物纳米粒子和二胺氧化酶或单胺氧化酶修饰的电极的安培生物胺生物传感器。

据报道，基于普鲁士蓝（PB）和铟锡氧化物纳米粒子（ITONP）修饰的丝网印刷碳电极（SPCE）的生物胺生物传感器。用二胺氧化酶（DAO）或单胺氧化酶（MAO）酶进一步修饰PB / ITONP修饰的SPCE，以构建生物传感器。通过扫描电子显微镜（SEM），能量色散X射线光谱（EDX）和原子力显微镜（AFM）研究了修饰电极的形貌。在生物传感器制造的每个步骤中，使用循环伏安法（CV）和电化学阻抗谱（EIS）来启发修饰电极的电化学性质。优化了电极表面组成和实验条件，研究了生物传感器的分析性能特征。测试了几种生物胺，两种生物传感器对组胺，腐胺和尸胺都有反应。DAO / ITONP / PB / SPCE生物传感器对组胺6.0×10-6-6.9×10-4 M的响应最高，灵敏度为1.84μAmM-1。另一方面，使用MAO / ITONP / PB / SPCE生物传感器获得的尸胺灵敏度最高。通过测定奶酪样品中的尸胺和组胺来说明所提出的生物传感器的分析实用性。