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Assessment of antioxidant system, cholinesterase activity and histopathology in Lithobates catesbeianus tadpoles exposed to water from an urban stream.
Ecotoxicology ( IF 2.7 ) Pub Date : 2020-02-27 , DOI: 10.1007/s10646-020-02176-6
Diego Romagna Boiarski 1 , Camila Maria Toigo 2 , Thaís Maylin Sobjak 3 , Andrey Felipe Potulski Santos 1 , Silvia Romão 4 , Ana Tereza Bittencourt Guimarães 1, 5
Affiliation  

Anthropogenic activities promote changes in community structure and decrease the species abundance of amphibians. The aim of this study was to assess potential alterations in the antioxidant system and cholinesterase activity, histopathological and oxidative damage in Lithobates catesbeianus tadpoles exposed to water from the Cascavel River, in Southern Brazil. Water samples (140 L each) were collected from the headwater, urban and rural areas of the river. Tadpoles were acclimated for seven days. After acclimatization tadpoles were reared in water from the river, except for the control aquarium. After seven days, a portion of the liver was removed and prepared for cholinesterase (ChE), superoxide dismutase (SOD), catalase (CAT) and lipid peroxidation (LPO) analysis; another part of the tissue was prepared for histological examination. An elevation of CAT activity was observed for water from both urban and rural environments. A decrease in LPO reaction was detected, mainly among the tadpoles exposed to water from the rural area. These alternations can cause delay the metamorphosis and lead to metabolic dysfunction, interfering in survival capacity and diminishing, not only individual fitness, but that of the whole population.

中文翻译:

暴露于城市河流水体中的石tho的抗氧化系统,胆碱酯酶活性和组织病理学评估。

人为活动促进了群落结构的变化,并减少了两栖动物的物种丰富度。这项研究的目的是评估巴西南部卡斯卡韦尔河中暴露于水中的石楠Li的抗氧化系统和胆碱酯酶活性,组织病理学和氧化损伤的潜在变化。从河源,城市和农村地区收集了水样(每个140升)。were适应了七天。驯化后,除了对照水族箱外,将from从河中饲养。7天后,取出一部分肝脏,准备进行胆碱酯酶(ChE),超氧化物歧化酶(SOD),过氧化氢酶(CAT)和脂质过氧化(LPO)分析。准备另一部分组织用于组织学检查。观察到城市和农村环境中的水的CAT活性均升高。发现LPO反应降低,主要是在暴露于农村地区水的the中。这些变化可能导致变态的延迟并导致代谢功能障碍,不仅影响个体的适应能力,而且影响整个人群的生存能力,并降低其生存能力。
更新日期:2020-02-28
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