Using biogenically synthesized silver nanoparticles (AgNPs) by Enterobacter cloacae Ism 26 (KP988024); Polyvinyl Alcohol-Silver (PVA-Ag) and Chitosan-Silver (CS-Ag) nanocomposites were prepared by augmentation. Thermal stability, AgNPs distribution and concentration within nanocomposites were characterized using thermogravimetric analysis (TGA), scanning electron microscope/energy dispersive x-ray (SEM/EDX) and atomic absorption spectroscopy (AAS). Nanocomposites films showed higher thermal stability than pure polymers, different AgNPs concentrations with homogenous distribution. Antibacterial, antibiofilm and cytotoxic activities of nanocomposites films were studied against Multi Drug Resistant (MDR) bacteria including Staphylococcus epidermidis (S.e), Staphylococcus aureus (S.a), Escherichia coli (E.c) and Klebsiella pneumoniae (K.p). PVA-Ag nanocomposite showed larger zones of inhibitions and higher antibiofilm activities than CS-Ag nanocomposite against both Gram-positive and Gram-negative bacteria. AgNPs solution, PVA-Ag and CS-Ag nanocomposites showed low cytotoxic activities against Huh-7 liver cells and IC₅₀ values of 263.20 μg ml⁻¹, 1185.07 μg ml⁻¹ and 119.85 μg ml⁻¹, respectively. PVA-Ag and CS-Ag nanocomposites films showed enhanced thermal stability and biological properties that qualify them for different biomedical applications.

使用阴沟肠杆菌Ism 26（KP988024）的生物合成的银纳米颗粒（AgNP）；通过增强制备聚乙烯醇银（PVA-Ag）和壳聚糖银（CS-Ag）纳米复合材料。使用热重分析（TGA），扫描电子显微镜/能量色散X射线（SEM / EDX）和原子吸收光谱（AAS）对纳米复合材料中的热稳定性，AgNPs分布和浓度进行了表征。纳米复合材料薄膜显示出比纯聚合物更高的热稳定性，不同的AgNPs浓度具有均匀的分布。研究了纳米复合材料薄膜对包括表皮葡萄球菌（Se）在内的耐多药（MDR）细菌的抗菌，抗生物膜和细胞毒性活性。，金黄色葡萄球菌（Sa），大肠杆菌（Ec）和肺炎克雷伯菌（Kp）。与CS-Ag纳米复合物相比，PVA-Ag纳米复合物对革兰氏阳性和革兰氏阴性细菌均显示出更大的抑制区域和更高的生物膜活性。的AgNPs溶液，PVA-Ag和CS-银纳米复合材料显示出低细胞毒性活性对的Huh-7肝细胞和IC _50个263.20微克毫升值^-1微克，1185.07毫升^-1和119.85微克毫升^-1分别。PVA-Ag和CS-Ag纳米复合材料薄膜显示出增强的热稳定性和生物特性，使其可用于不同的生物医学应用。