The prediction of the performance of a Centrifugal Partition Chromatography (CPC) is a difficult but desirable task. The partitioning of the sample, as well as the fluid dynamical phenomena dispersion, coalescence, and stationary phase retention have to be individually understood. Therefore, the phase settling behavior of different aqueous-organic solvent systems and with this, the dependency of the stationary phase retention in CPC was investigated in this study. On the one hand, batch settling experiments were performed, and the settling velocity of aqueous-organic solvent systems was investigated. With this it was possible to correlate the stationary phase retention in CPC in both operating modes. For descending mode operation a high settling velocity of the lower phase and for ascending mode a high settling velocity of the upper phase is needed for a stable operation and a high stationary phase retention. On the other hand, the dimensionless numbers Capillary number (Ca) and Morton number (Mo) were used to generate a universally applicable correlation for the stationary phase retention in ascending mode. It was shown, that a high stationary phase retention correlates with low values of Ca and Mo, whereas the influence of Mo is neglectable in the parameter space investigated. Within this correlation, the individual influence of each influencing parameter on the stationary phase retention was included. Moreover, this correlation was compared to descriptions for descending mode given in literature. With this it was shown that the minimal stationary phase retention is correlatable to the point of phase inversion.

中文翻译：

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关联水性有机溶剂系统在离心分离色谱仪中的相沉降行为。

离心分离色谱法（CPC）的性能预测是一项困难但理想的任务。必须分别了解样品的分配以及流体动力学现象的分散，聚结和固定相保留。因此，在这项研究中，研究了不同的水性有机溶剂体系的相沉降行为，并以此来研究固定相在CPC中保留的依赖性。一方面，进行了间歇沉降实验，并研究了有机溶剂水溶液的沉降速度。这样，就可以将两种操作模式下CPC中的固定相保持率关联起来。对于下降模式操作，较低相的高沉降速度；对于上升模式，需要较高相的上沉降速度，以实现稳定的操作和较高的固定相保持率。另一方面，使用无量纲数的毛细管数（Ca）和莫顿数（Mo）来生成普遍适用的相关性，以用于升模下的固定相保留。结果表明，较高的固定相保留与较低的Ca和Mo值相关，而在所研究的参数空间中Mo的影响可忽略不计。在这种关系中，包括了每个影响参数对固定相保留的单独影响。此外，将这种相关性与文献中对下降模式的描述进行了比较。