Maintaining a healthy proteome throughout life is critical for proper somatic stem cell function, but the complexities of the stem cell response to increases in damaged or aggregated proteins remain unclear. Here we demonstrate that adult neural stem cells (NSCs) utilize aggresomes to recover from disrupted proteostasis and describe a novel function for the intermediate filament vimentin in proteostasis as a spatial coordinator of proteasomes to the aggresome. In the absence of vimentin, NSCs have a reduced capacity to exit quiescence, a time when NSCs are required to clear a wave of aggregated proteins, and demonstrate an early age-dependent decline in proliferation and neurogenesis. Taken together, these data reveal a significant role of vimentin and aggresomes in the regulation of proteostasis during quiescent NSC activation.

中文翻译：

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波形蛋白在神经干细胞静止退出期间协调聚集体的蛋白质周转。

终生保持健康的蛋白质组对于体细胞干细胞的正常功能至关重要，但干细胞对受损或聚集蛋白质增加的反应的复杂性仍不清楚。在这里，我们证明了成体神经干细胞 (NSC) 利用聚集体从破坏的蛋白质稳态中恢复，并描述了蛋白质稳态中中间丝波形蛋白作为蛋白酶体与聚集体的空间协调器的新功能。在没有波形蛋白的情况下，NSCs 退出静止状态的能力降低，此时 NSCs 需要清除一波聚集蛋白，并表现出增殖和神经发生的早期年龄依赖性下降。总之，这些数据揭示了波形蛋白和聚集体在静止 NSC 激活期间在调节蛋白质稳态中的重要作用。