Growth differentiation factor 11 (GDF11) is a TGF‐β superfamily circulating factor that regulates cardiomyocyte size in rodents, sharing 90% amino acid sequence identity in the active domains with myostatin (GDF8)—the major determinant of skeletal muscle mass. Conflicting data on age‐related changes in circulating levels have been reported mainly due to the lack of specific detection methods. More recently, liquid chromatography tandem mass spectrometry (LC‐MS/MS) based assay showed that the circulating levels of GDF11 do not change significantly throughout human lifespan, but GDF8 levels decrease with aging in men. Here a novel detection method is demonstrated based on parallel reaction monitoring LC‐MS/MS assay combined with immunoprecipitation to reliably distinguish GDF11 and GDF8 as well as determine their endogenous levels in mouse serum. The data indicate that both GDF11 and GDF8 circulating levels significantly decline with aging in female mice.

中文翻译：

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区分和确定小鼠血清中GDF8和GDF11绝对水平的靶向方法。

生长分化因子11（GDF11）是一种TGF-β超家族循环因子，可调节啮齿动物的心肌细胞大小，并在与肌生长抑制素（GDF8）（即骨骼肌质量的主要决定因素）的活跃域中共享90％的氨基酸序列同一性。据报道，与年龄相关的血液循环水平变化的数据相互矛盾，这主要是由于缺乏特定的检测方法。最近，基于液相色谱串联质谱（LC-MS / MS）的测定表明，GDF11的循环水平在整个人类寿命中没有显着变化，但是GDF8的水平随着男性的衰老而降低。本文在平行反应监测LC-MS / MS分析与免疫沉淀相结合的基础上展示了一种新颖的检测方法，以可靠地区分GDF11和GDF8并确定其在小鼠血清中的内源性水平。