Cys is a common and important sulfur-containing amino acid in living organisms, whose intracellular level changes are associated with a variety of diseases. In order to specifically detect Cys without interference from other thiol species, we have developed a rhodol-enone dye platform (termed as probe BL-C) with an acrylate group and an α,β-unsaturated ketone as bis-reaction-triggers. Our probe BL-C can remarkably exhibit a turn on signal towards Cys with high selectivity. Moreover, it has been successfully applied for detection of cysteine in living cells in terms of its excellent cell permeability. In conclusion, these desirable characteristics indicate that probe BL-C could be applied to discriminative sensing of intracellular Cys in biological applications.

中文翻译：

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具有双重反应触发器的Rhodol-enone染料平台，可特异性检测Cys。

半胱氨酸是活生物体中一种常见且重要的含硫氨基酸，其胞内水平变化与多种疾病有关。为了在不受到其他硫醇种类干扰的情况下特异性检测Cys，我们开发了具有丙烯酸酯基团和α，β-不饱和酮作为双反应触发器的Rhodol-烯酮染料平台（称为探针BL-C）。我们的探针BL-C可以以高选择性显着地向Cys展示开启信号。此外，就其优异的细胞渗透性而言，它已成功地用于检测活细胞中的半胱氨酸。总之，这些理想的特性表明，探针BL-C可用于生物学应用中的细胞内Cys的判别感测。