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A Combinatorial MAP Code Dictates Polarized Microtubule Transport.
Developmental Cell ( IF 11.8 ) Pub Date : 2020-02-27 , DOI: 10.1016/j.devcel.2020.01.029
Brigette Y Monroy 1 , Tracy C Tan 1 , Janah May Oclaman 1 , Jisoo S Han 2 , Sergi Simó 2 , Shinsuke Niwa 3 , Dan W Nowakowski 4 , Richard J McKenney 1 , Kassandra M Ori-McKenney 1
Affiliation  

Many eukaryotic cells distribute their intracellular components asymmetrically through regulated active transport driven by molecular motors along microtubule tracks. While intrinsic and extrinsic regulation of motor activity exists, what governs the overall distribution of activated motor-cargo complexes within cells remains unclear. Here, we utilize in vitro reconstitution of purified motor proteins and non-enzymatic microtubule-associated proteins (MAPs) to demonstrate that MAPs exhibit distinct influences on the motility of the three main classes of transport motors: kinesin-1, kinesin-3, and cytoplasmic dynein. Further, we dissect how combinations of MAPs affect motors and unveil MAP9 as a positive modulator of kinesin-3 motility. From these data, we propose a general "MAP code" that has the capacity to strongly bias directed movement along microtubules and helps elucidate the intricate intracellular sorting observed in highly polarized cells such as neurons.

中文翻译:

组合MAP代码指示极化的微管运输。

许多真核细胞通过分子马达沿着微管轨道驱动的调控主动转运,不对称地分布其细胞内成分。虽然存在内在和外在的运动活动调节,但是控制细胞内活化的运动-货物复合物的总体分布的因素仍然不清楚。在这里,我们利用纯化的运动蛋白和非酶微管相关蛋白(MAPs)的体外重组来证明MAPs对三种主要的运动运输蛋白的运动性具有不同的影响:驱动蛋白1,驱动蛋白3和运动蛋白。细胞质动力蛋白。此外,我们分析了MAP的组合如何影响电机,并揭示了MAP9作为驱动蛋白3的正向调节剂。根据这些数据,我们提出了一个通用的“ MAP代码”
更新日期:2020-02-27
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