Eukaryotic genomes are organized within the nucleus through interactions with inner nuclear membrane (INM) proteins. How chromatin tethering to the INM is controlled in interphase and how this process contributes to subsequent mitotic nuclear envelope (NE) remodeling remains unclear. We have probed these fundamental questions using the fission yeast Schizosaccharomyces japonicus, which breaks and reforms the NE during mitosis. We show that attachments between heterochromatin and the transmembrane Lem2-Nur1 complex at the INM are remodeled in interphase by the ESCRT-III/Vps4 machinery. Failure of ESCRT-III/Vps4 to release Lem2-Nur1 from heterochromatin leads to persistent association of chromosomes with the INM throughout mitosis. At mitotic exit, such trapping of Lem2-Nur1 on heterochromatin prevents it from re-establishing nucleocytoplasmic compartmentalization. Our work identifies the Lem2-Nur1 complex as a substrate for the nuclear ESCRT machinery and explains how the dynamic tethering of chromosomes to the INM is linked to the establishment of nuclear compartmentalization.

中文翻译：

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ESCRT-III/Vps4 控制异染色质-核包膜附件。

真核基因组通过与内核膜 (INM) 蛋白的相互作用在细胞核内组织。在间期如何控制染色质与 INM 的束缚以及该过程如何促进随后的有丝分裂核膜 (NE) 重塑仍不清楚。我们已经使用裂殖酵母 Schizosaccharomyces japonicus 探讨了这些基本问题，它在有丝分裂过程中破坏和改造 NE。我们表明，异染色质和 INM 处的跨膜 Lem2-Nur1 复合物之间的连接在间期被 ESCRT-III/Vps4 机器改造。ESCRT-III/Vps4 未能从异染色质中释放 Lem2-Nur1 导致染色体在有丝分裂过程中与 INM 持续结合。在有丝分裂出口处，Lem2-Nur1 在异染色质上的这种捕获阻止了它重新建立核质区室化。我们的工作将 Lem2-Nur1 复合物确定为核 ESCRT 机制的底物，并解释了染色体与 INM 的动态束缚如何与核区室化的建立相关联。