Litopenaeus vannamei, as an important marine aquaculture species, has attracted more and more attentions in past several years. More recently people got its genome fine mapping, which unveiled a gene treasure. In this study, we have identified a novel trypsin-like protein which came from previous WSSV-infected shrimp plasma iTRAQ data. This protein is a 39 kDa protein with 363 amino acids. It contains a conserved trypsin-domain and could be strongly induced with WSSV infection. Interestingly, knockdown of this protein made shrimps vulnerable to WSSV infection. Further exploration unveiled that this fragility was probably due to the fact that knockdown of this protein could cause shrimp hemocytes apoptosis, which indicated that this protein played key roles in preventing shrimp hemocytes from apoptosis. To further explore how LvTLAP protected shrimp hemocytes from apoptosis, GST pull down assay was applied to screen LvTLAP interacting protein in shrimp plasma. L. vannamei growth and transformation-dependent-like protein (LvGTD-like protein) was identified as a LvTLAP interacting protein, which played proapoptotic roles in cells. Thus, a possible explanation for LvTLAP anti-apoptosis activity was that this protein could block LvGTD-like protein proapoptotic activity to protect shrimp hemocytes from death. In general, our study has uncovered a novel WSSV responsive shrimp plasma protein, which played key roles in shrimp hemocytes anti-apoptosis and shrimp against WSSV infection.

中文翻译：

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凡纳滨对虾中一种新型的WSSV响应血浆蛋白有助于血细胞抗凋亡。

南美白对虾作为重要的海水养殖品种，在过去几年中受到越来越多的关注。最近，人们对其基因组进行了精细定位，从而揭示了基因宝藏。在这项研究中，我们从以前的WSSV感染的虾血浆iTRAQ数据中鉴定了一种新型的胰蛋白酶样蛋白。该蛋白是具有363个氨基酸的39 kDa蛋白。它包含一个保守的胰蛋白酶域，并可能被WSSV感染强烈诱导。有趣的是，这种蛋白质的敲低使虾容易受到WSSV感染。进一步的探索表明，这种脆弱性可能是由于该蛋白的敲低可能导致虾血细胞凋亡的事实所致，这表明该蛋白在防止虾血细胞凋亡中起关键作用。为了进一步探索LvTLAP如何保护虾血细胞免于凋亡，采用GST下拉检测法筛选虾血浆中的LvTLAP相互作用蛋白。南美白对虾生长和转化依赖性蛋白（LvGTD-like蛋白）被鉴定为LvTLAP相互作用蛋白，在细胞中发挥促凋亡作用。因此，LvTLAP抗凋亡活性的可能解释是该蛋白可以阻断LvGTD样蛋白的凋亡活性，从而保护虾血细胞免于死亡。总的来说，我们的研究发现了一种新型的WSSV反应性虾血浆蛋白，该蛋白在虾血细胞抗凋亡和虾抗WSSV感染中起关键作用。南美白对虾生长和转化依赖性蛋白（LvGTD-like蛋白）被鉴定为LvTLAP相互作用蛋白，在细胞中发挥促凋亡作用。因此，LvTLAP抗凋亡活性的可能解释是该蛋白可以阻断LvGTD样蛋白的凋亡活性，从而保护虾血细胞免于死亡。总的来说，我们的研究发现了一种新型的WSSV反应性虾血浆蛋白，该蛋白在虾血细胞抗凋亡和虾抗WSSV感染中起关键作用。南美白对虾生长和转化依赖性蛋白（LvGTD-like蛋白）被鉴定为LvTLAP相互作用蛋白，在细胞中发挥促凋亡作用。因此，LvTLAP抗凋亡活性的可能解释是该蛋白可以阻断LvGTD样蛋白的凋亡活性，从而保护虾血细胞免于死亡。总的来说，我们的研究发现了一种新型的WSSV反应性虾血浆蛋白，该蛋白在虾血细胞抗凋亡和虾抗WSSV感染中起关键作用。