Background

Anthocyanins, one of the major plant bioactive substances, possess anti-oxidative and anti-inflammatory capacity. However, their dose–response relationship has remained unclear. The present study investigated the dose–response relationship of anthocyanins with oxidative stress and inflammation in subjects with dyslipidemia.

Design

and Participants: A total of 169 participants with dyslipidemia were randomly assigned to placebo (n = 43), anthocyanins 40 mg/day (n = 44), 80 mg/day (n = 40), or 320 mg/day (n = 42) groups. Urine 8-iso-prostaglandin F_2α (8-iso-PGF_2α), 8-hydroxy-2′-deoxyguanosine (8-OHdG) and serum malonaldehyde (MDA), total superoxide dismutase (T-SOD), UA (uric acid), interleukin (IL)-6, IL-10, tumor necrosis factor-α (TNF-α), and C-reactive protein (CRP) were measured at baseline, at 6 weeks, and at 12 weeks.

Results

Anthocyanin supplementation (320 mg/day) for 6 weeks significantly improved T-SOD versus baseline (P < 0.05). A slight reduction in serum IL-6, TNF-α, and urine 8-iso-PGF_2α from the baseline was observed at 12 weeks in the group receiving 40 mg/day anthocyanins. Anthocyanins (80 mg/day) significantly reduced serum IL-6 (−20%), TNF-α (−11%) and urine 8-iso-PGF_2α (−27%) versus baseline (P < 0.05). Moreover, 320 mg/day anthocyanin supplementation reduced serum IL-6 (−40%), TNF-α (−21%), MDA (−20%) and urine 8-iso-PGF_2α (−37%) and 8-OHdG (−36%) than 80 mg/day and 40 mg/day anthocyanins, P value < 0.05. Anthocyanin supplementation has dose-response relationships with decreased inflammatory cytokines IL-6, TNF-α and oxidative stress biomarkers 8-iso-PGF_2α, 8-OHdG and MDA (P for trend, <0.05). Furthermore, a strong positive correlation was observed between the changes in the urine 8-iso-PGF_2α , 8-OHdG levels and serum IL-6 levels in subjects from anthocyanin groups after 12 weeks of treatment.

Conclusions

Supplementation of anthocyanins for 12 weeks positively improved the anti-oxidative and anti-inflammatory capacity in a dose–response manner in individuals with dyslipidemia.

中文翻译：

---

补充花青素可以剂量反应的方式提高血脂异常受试者的抗氧化和抗炎能力。

背景

花青素是主要的植物生物活性物质之一，具有抗氧化和抗炎能力。然而，它们的剂量-反应关系仍不清楚。本研究调查了花青素与血脂异常受试者氧化应激和炎症的剂量反应关系。

设计

参与者：共有 169 名患有血脂异常的参与者被随机分配到安慰剂组 ( n  = 43)、花青素 40 毫克/天 ( n  = 44)、80 毫克/天 ( n  = 40) 或 320 毫克/天 ( n  = 42) 团体。尿液8-异前列腺素F _{2 α} (8-iso-PGF _{2 α} )、8-羟基-2'-脱氧鸟苷(8-OHdG)和血清丙二醛(MDA)、总超氧化物歧化酶(T-SOD)、UA (在基线、6周和12周时测量白细胞介素(IL)-6、IL-10、肿瘤坏死因子-α (TNF- α )和C反应蛋白(CRP)。

结果

与基线相比，补充花青素（320 毫克/天）6 周显着改善 T-SOD（P  < 0.05）。在接受 40 mg/天花青素的组中，第 12 周时观察到血清 IL-6、TNF- α和尿液 8-iso-PGF _{2 α}较基线略有下降。与基线相比，花青素（80 mg/天）显着降低血清 IL-6 (-20%)、TNF- α (-11%) 和尿液 8-iso-PGF _{2 α} (-27%)（P  < 0.05）。此外，每天补充 320 毫克花青素可降低血清 IL-6 (-40%)、TNF- α (-21%)、MDA (-20%) 和尿液 8-iso-PGF _{2 α} (-37%) 和 8 -OHdG (−36%) 高于 80 毫克/天和 40 毫克/天花青素，P值 < 0.05。补充花青素与炎症细胞因子 IL-6、TNF- α和氧化应激生物标志物 8-iso-PGF _{2 α}、8-OHdG 和 MDA的减少具有剂量反应关系（趋势P <0.05）。此外，治疗12周后，花青素组受试者尿液8-iso-PGF _{2 α} 、8-OHdG水平与血清​​IL-6水平的变化之间观察到强正相关。

结论

补充花青素 12 周可以剂量反应方式积极改善血脂异常个体的抗氧化和抗炎能力。