Abstract The composite filler micro-embedded with Pseudomonas putida (P. putida) was prepared and the biodegradation performance of the filler was evaluated in a biofilter. Five phases were set up to evaluate the performance of the biofilter under different toluene inlet loadings and transient shock loadings. In particular, the microbial community structure in the biofilms and fillers was measured by sequence analysis of the 16S rRNA gene. The results show that the biofilter packed with the composite fillers was suitable for the biodegradation of toluene. The biofilter could start up quickly with high removal efficiency (RE), and remain above 90 % RE when the empty bed residence time (EBRT) was 18 s and the inlet loading rates were not higher than 41.4 g/(m3·h). Moreover, the biofilter could tolerate substantial transient shock loadings. The high removal efficiency and elimination capacity contributed to rich bacterial communities for the efficient degradation of toluene. The dominant microbial communities at the phylum level were mainly Firmicutes, Actinobacteria and Proteobacteria. It is noteworthy that the abundance of Bacteroidetes at phylum level and Chungangia and Stenotrophomonas at genus level increased significantly during the re-start period.

中文翻译：

---

恶臭假单胞菌微包埋复合填料降解甲苯的性能评价及微生物群落分析

摘要 制备了恶臭假单胞菌（P. putida）微包埋复合填料，并在生物滤池中对填料的生物降解性能进行了评价。设置了五个阶段来评估生物过滤器在不同甲苯入口负载和瞬态冲击负载下的性能。特别是，生物膜和填料中的微生物群落结构是通过 16S rRNA 基因的序列分析来测量的。结果表明，填充复合填料的生物滤池适用于甲苯的生物降解。生物滤池启动快，去除率高，空床停留时间（EBRT）为18 s，入口负荷率不高于41.4 g/(m3·h)时，可保持90%以上的去除率。此外，生物过滤器可以承受大量的瞬态冲击载荷。高去除效率和消除能力有助于丰富的细菌群落，以有效降解甲苯。门水平的优势微生物群落主要是厚壁菌门、放线菌门和变形菌门。值得注意的是，在重新启动期间，门水平的拟杆菌和属水平的春根藻和狭养单胞菌的丰度显着增加。