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Cryo-imaging of Stem Cell Biodistribution in Mouse Model of Graft-Versus-Host-Disease.
Annals of Biomedical Engineering ( IF 3.8 ) Pub Date : 2020-02-26 , DOI: 10.1007/s10439-020-02487-z
Patiwet Wuttisarnwattana 1, 2 , Saada Eid 3 , Madhusudhana Gargesha 4 , Kenneth R Cooke 5 , David L Wilson 6
Affiliation  

We demonstrated the use of multispectral cryo-imaging and software to analyze human mesenchymal stromal cells (hMSCs) biodistribution in mouse models of graft-versus-host-disease (GVHD) following allogeneic bone marrow transplantation (BMT). We injected quantum dot labeled MSCs via tail vein to mice receiving BMT and analyzed hMSC biodistribution in major organs (e.g. lung, liver, spleen, kidneys and bone marrow). We compared the biodistribution of hMSCs in mice following allogeneic BMT recipients (with GVHD) to the biodistribution following syngeneic BMT (without GVHD). Cryo-imaging system revealed cellular biodistribution and redistribution patterns in the animal model. We initially found clusters of cells in the lung that eventually dissociated to single cells and redistributed to other organs within 72 h. The in vivo half-life of the exogenous MSCs was about 21 h. We found that the biodistribution of stromal cells was not related to blood flow, rather cells preferentially homed to specific organs. In conclusion, cryo-imaging was suitable for analyzing the cellular biodistribution. It could provide capabilities of visualizing cells anywhere in the mouse model with single cell sensitivity. By characterizing the biodistribution and anatomical specificity of a therapeutic cellular product, we believe that cryo-imaging can play an important role in the advancement of stem and stromal cell therapies and regenerative medicine.

中文翻译:

移植物抗宿主宿主疾病小鼠模型中干细胞生物分布的低温成像。

我们展示了多光谱冷冻成像和软件的使用,以分析同种异体骨髓移植(BMT)后移植物抗宿主疾病(GVHD)小鼠模型中的人间充质基质细胞(hMSCs)生物分布。我们通过尾静脉向接受BMT的小鼠注射了量子点标记的MSC,并分析了hMSC在主要器官(如肺,肝,脾,肾和骨髓)中的生物分布。我们比较了同种BMT受体(GVHD)后小鼠中hMSCs的生物分布与同基因BMT(无GVHD)后小鼠中hMSCs的生物分布。冷冻成像系统揭示了动物模型中细胞的生物分布和再分布模式。我们最初发现肺中的细胞簇最终在72小时内解离为单个细胞并重新分布到其他器官。外源MSC的体内半衰期为约21小时。我们发现基质细胞的生物分布与血流无关,而是细胞优先归巢于特定器官。总之,冷冻成像适用于分析细胞的生物分布。它可以提供具有单个细胞敏感性的可视化鼠标模型中任何位置的细胞的功能。通过表征治疗性细胞产品的生物分布和解剖学特异性,我们相信冷冻成像可以在干细胞和基质细胞疗法以及再生医学的发展中发挥重要作用。冷冻成像适用于分析细胞的生物分布。它可以提供具有单个细胞敏感性的可视化鼠标模型中任何位置的细胞的功能。通过表征治疗性细胞产品的生物分布和解剖学特异性,我们相信冷冻成像可以在干细胞和基质细胞疗法以及再生医学的发展中发挥重要作用。冷冻成像适用于分析细胞的生物分布。它可以提供具有单个细胞敏感性的可视化鼠标模型中任何位置的细胞的功能。通过表征治疗性细胞产品的生物分布和解剖学特异性,我们相信冷冻成像可以在干细胞和基质细胞疗法以及再生医学的发展中发挥重要作用。
更新日期:2020-02-26
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